E-Locus - Institutional Repository of the University of Crete

Home Collections School/Department School of Medicine Department of Medicine Post-graduate theses

Post-graduate theses

Search command : Author="Μπριασούλης" And Author="Γεώργιος"

Current Record: 723 of 1248

[Add to Basket]

Identifier

000396303

Title

Scructure and function of the circuits underlying reward

Alternative Title

Δομή και λειτουργία των κυκλωμάτων που υπόκεινται της ανταμοιβής

Author

Τζώρτζη, Ουρανία

Thesis advisor

Meletis, Dinos

Reviewer

Maritina, Sergaki
Iakovos, Lazaridis
Maria, Papathanou

Abstract

Lateral habenula (LHb) is in a unique position to process reward-related signals into proper behavioral responses, particularly during aversive, stressful situations. Its inhibitory effects upon dopamine rich areas, indicate the key role of LHb in the modulation of brain reward system. Excitatory inputs from LHb target GABAergic interneurons of both ventral tegmental area (VTA) and rostromedial tegmental nucleus (RMTg) and drive strong inhibition on dopaminergic neurons of VTA. These reward-related signals are provided to LHb from distinct populations arising from Globus Pallidus internus (GPi). However, the nature and potential regulation of the major input to LHb originating from the basal ganglia, is poorly understood. Here by using a dual viral combination of an AAV helper virus and a genetically modified rabies virus that displays specific transynaptic retrograde spread (1) we are providing anatomical evidence for a strong innervation of LHb, by VGluT2+ glutamatergic and VGaT+ GABAergic GPi neurons. (2) We also demonstrate that the former VGluT2+ LHb projecting GPi neurons receive direct monosynaptic inputs arising from the matrix and patch compartments of the caudate putamen (CPu) as well as the prototypic neurons of the Globus Pallidus externus. (3) Furthermore, we show that the optogenetic excitation of the VGluT2+ LHb projecting GPi inputs promotes a strong aversive behavior. Taken the importance of LHb as a modulatory nucleus of the dopaminergic system, the dissection of its connectivity and function will give valuable insights in the understanding of both reward-seeking behavior and depressive disorders.

Language

English

Subject

Habenusa

Πλευρική ηνία

Issue date

2015-07-17

Collection

School/Department--School of Medicine--Department of Medicine--Post-graduate theses

Type of Work--Post-graduate theses

Permanent Link

https://elocus.lib.uoc.gr//dlib/c/2/0/metadata-dlib-1442394699-822535-5098.tkl