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Identifier

000465537

Title

The Achaete-Scute Complex vs Notch signaling in Drosophila embryonic neurogenesis : New insigh

Alternative Title

Το σύμπλοκο Achaete-Scute ενάντια στη Notch σηματοδότηση κατά την εμβρυική νευρογένεση στη Δροσόφιλα

Author

Σούκουλη, Ιωάννα

Thesis advisor

Δελιδάκης, Χρήστος
Θεοδώρου, Βασιλική

Reviewer

Μοναστηριώτη, Μαρία
Παπαδόπουλος, Δημήτριος

Abstract

The development of the embryonic nervous system in Drosophila is regulated by a complex mechanism and begins with the generation of neural progenitors, called neuroblasts (NBs). The two key players that participate in this process are the Achaete-Scute Complex (ASC) and the Notch (N) signaling pathway. The ASC promotes the neural fate, while the N pathway represses it and promotes the epidermal one, through a process called lateral inhibition. The interactions between these two, as well as other transcription factors (TFs) in embryonic neurogenesis remain unclear and their understanding could clarify this complicated regulatory network. In this study, we examine several TFs that are targets of the ASC. First, we study neuroectodermal TFs’ expression patterns and their dependency on the ASC in wild type and in mutant embryos. Secondly, we examine the phenotype of their loss-of-function mutations on neurogenesis and lastly we over-express epidermal TFs in N deficiency asking if this could reverse the hypertrophic nervous system caused by N deficiency. The results suggest that neuroectodermal TFs are independent of the ASC, even though they are targeted by it. The experiments with loss-of-function mutations indicate genes, such as Kr, with a possibly redundant action to the ASC in neurogenesis. The overexpression of epidermal TFs in N deficient was only minimally able to improve the neural hyperplasia of the latter. It is suggested that the network that regulates embryonic neurogenesis includes ASC, N pathway and other genes participating in NB formation, as shown in this study and there is evidence that they co-operate during development.

Language

English

Subject

Neuroblasts

Proneural genes

Transcription factors

Μεταγραφικοί παράγοντες

Νευροβλάστες

Προνευρικά γονίδια

Issue date

2024-07-26

Collection