Post-graduate theses
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Identifier |
000376879 |
Title |
Μελέτη της επίδρασης των πρωτεινών θερμικού πλήγματος 70(Ηsp70) στο στρέσ |
Alternative Title |
The role of Hsp70 proteins during stress |
Author
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Γεωργοπούλου, Ανθή
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Thesis advisor
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Βενυχάκη, Μαρία
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Abstract |
Hypothalamic‐pituitary adrenal (HPA) axis is the main endocrine axis regulating the stress
response including inflammation. Heat Shock protein (HSP) expression is vital to cellular and
tissue protection after stress or injury. The 70‐kDa heat shock protein (HSP70) family is a
group of proteins that are critical for protein assembly, folding, and transport. Furthermore,
HSP70 are involved in the inflammatory stress. Based on the above, the aim of the present
study was to evaluate the role of HSP70 on the hypothalamic‐pituitary adrenal (HPA) axis
during stress. For our studies we used the Hsp70 deficient mice (Hsp70‐/‐) and their wildtype
littermates (Hsp70+/+) which were subjected to restraint and inflammatory pain. Our results
showed that Hsp70‐/‐ mice had lower levels of CRH and POMC mRNA levels compared to
Hsp70+/+ mice under basal conditions and following restraint for 3 hrs. However, plasma
glucocorticoid levels did not differ between the two genotypes under basal conditions and
following stress. Similarly no differences were observed between the two genotypes
following 15 min of restraint. Moreover, six hours after injection with CFA, pain threshold
was reduced in Hsp70‐/‐ mice in contrast to wildtype mice. Corticosterone levels, at the
same time point, were reduced in Hsp70 ‐/‐ mice compared to their wildtype littermates.
Our results indicate a role of HSP70 on the activation of the HPA axis during stress. The
mechanism of action of HSP70 on the HPA axis activity is under investigation.
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Language |
Greek |
Subject |
Biochemistry |
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Flammatory pain |
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HPA axis |
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Hsp70 family |
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Restraint experimental |
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Πειράματα περιορισμού |
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Φλεγμονώδης πόνος |
Issue date |
2012-12-11 |
Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/b/a/d/metadata-dlib-1354014099-705580-21605.tkl
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Views |
178 |