Post-graduate theses
Current Record: 5 of 1207
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Identifier |
000461081 |
Title |
Deciphering molecular regulators of neuroinflammation in Alzheimers disease using iPSC-derived microglia |
Alternative Title |
Αποκωδικοποιώντας τους μοριακούς ρυθμιστές της νευροφλεγμονής στη νόσο Αλτσχάιμερ με τη χρήση επαγόμενων πολυδύναμων βλαστικών κυττάρων μικρογλοίας |
Author
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Λυγερούδη, Ανίτα
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Thesis advisor
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Σάλτα, Ευγενία
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Reviewer
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Penning, Amber
Snoeck, Sarah
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Abstract |
Alzheimer’s disease (AD) is a fatal neurodegenerative disease with no available treatment.
Since the generation of induced pluripotent stem cells (iPSCs), several cell-based models of
different diseases have been developed. In an attempt to better understand the disease aetiology
and identify effective therapeutics for AD, human iPSC lines have been utilised to study both
the familial (fAD) and the sporadic (sAD) form of AD. However, there has not yet been a
systematic and detailed characterization of microglia derived from human sAD iPSC lines at
baseline. By monitoring gene expression profiles using quantitative PCR and
immunofluorescent labelling, we focus on the characterization of two iPSC lines derived from
healthy individuals and one iPSC line derived from a patient with sAD. We found that iPSCderived
microglia highly express microglial markers, thereby validating their cellular identity
in our experimental setting. In addition, we showed an upregulation of DAM and AD risk genes
in the sAD iPSC line, and we observed distinct morphological characteristics in the sAD iPSC
line compared to controls, suggestive of a more activated phenotype, recapitulating microglial
behavior in AD brain. Interestingly, despite these observations no downregulation of miR-132
expression levels in the sAD iPSC line was observed. This study will provide fundamental
knowledge for future reference, to better evaluate prospective results under different treatment
conditions.
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Language |
English |
Subject |
Baseline characterisation |
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Induced pluripotent stem-cells |
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Sporadic Alzheimers disease |
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Σποραδική νόσος Αλτσαχάιμερ |
Issue date |
2023-12-08 |
Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/3/6/7/metadata-dlib-1702036009-538872-21299.tkl
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Views |
1127 |