Post-graduate theses
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Identifier |
000389804 |
Title |
Μελέτη των προγονικών ενδοθηλιακών κυττάρων σε νέους ασθενείς με σακχαρώδη διαβήτη τύπου Ι |
Alternative Title |
Study of endothelial progenitor cells in young patients with diabetes mellitus Type I |
Author
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Σημαντηράκη, Δέσποινα Ε.
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Thesis advisor
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Τσιλιμπάρης, Μιλτιάδης
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Reviewer
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Παπαδάκη, Ελένη
Γανωτάκης, Εμμανουήλ
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Abstract |
Purpose: To evaluate the presence of Endothelial Progenitor Cells (EPCs) in the peripheral blood
of young patients with Diabetes Mellitus Type I (T1DM).
Method & Material: Blood samples were collected and analyzed from 69 individuals (mean ageyears:
27, range:8-50): 39 patients with DM (mean DM duration-years: 16 (range:2-31)), mean
age-years: 26(8-50) and 30 controls without DM mean age-years: 28 (range: 9-45). The presence
of Diabetic Retinopathy (DR) was documented with slit lamp examination and fluorescence
angiography, according to the Early Treatment of Diabetic Retinopathy Study (ETDRS) criteria.
The EPCs were detected with flow cytometry using the following primary antibodies: anti-CD34,
anti-CD45, anti-CD133 and anti-hVEGFR2.
Results: Progenitor EPCs, defined as CD45-/CD34+/CD133+/VEGFR2+ cells, were detected in
31/69 samples (mean%: 0.00042, SEM:0.00007). 20/39 patients had EPCs detected in the
periphery blood (mean%: 0.00054, SEM:0.00014). EPCs were detected in 11/15 patients with
PDR (mean%: 0.00061, SEM:0.00013). Also, 4/9 patients with NPDR (mean%: 0.00071,
SEM:0.00039) and 5/15 patients without clinically proven DR (mean%: 0.00038, SEM:0.00026)
had detectable EPCs. Finally, circulating EPCs were identified in 11/30 individuals of the control
group (mean%: 0.00026, SEM:0.00007).
Conclusions: CD45-/CD34+/CD133+/hVEGFR2+ EPCs were detected in the peripheral blood of
non diabetic persons and diabetic patients with or without evidence of DR. The presence of EPCs
in the peripheral blood of young diabetics seem to correlate with the DR stage. Study of EPCs in
these patients could offer significant information about the pathophysiology of diabetic
microangiopathy.
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Language |
Greek |
Subject |
Diabetes Mellitus Type I |
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Endothelial progenitor cells |
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Προγονικά ενδοθυλιακά κύτταρα |
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Σακχαρώδης Διαβήτης Τύπου Ι |
Issue date |
2015-03-31 |
Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/4/3/f/metadata-dlib-1423755701-11881-30883.tkl
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Views |
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