Your browser does not support JavaScript!

Home    Collections    School/Department    School of Medicine    Department of Medicine    Doctoral theses  

Doctoral theses

Current Record: 8 of 968

Back to Results Previous page
Next page
Add to Basket
[Add to Basket]
Identifier 000432491
Title Μελέτη της έκφρασης και του βιολογικού ρόλου των μορίων συνάφειας (integrins , cadherins ) στα ωάρια και έμβρυα ποντικών μετά απο υπερδιέγερση ωοθηκών και επίδραση με L-carnitine
Alternative Title Study of expression and biological role of adhesion molecules (integrins ,cadherins ) in mice eggs and embryos after ovarian over stimulation and effect of L-carnitine
Author Ανυφαντάκη, Αλίκη
Thesis advisor Ζαφειρόπουλος, Αλέξανδρος
Reviewer Αθανασάκη, Ειρήνη
Τσατσάνης, Χρήστος
Θεοδωρόπουλος, Παναγιώτης
Ματαλλιωτάκης, Ιωάννης
Χαλεπάκης, Γεώργιος
Σουρβίνος, Γεώργιος
Abstract Despite the improvements in technology and know-how in human assisted reproduction, pregnancy rates remain low due to an imbalanced interaction between the embryo and the uterus upon ovarian stimulation. The normal reproductive process is controlled by a set of interactions that take place either among cells or between cells and the extracellular matrix (ECM). Such interactions involve cells, ligands, cell adhesion molecules, adhesion molecules that mediate cell-to-cell and cell-to-ECM interactions, as well as their receptors. The most important groups of these receptors are integrins and cadherins, which are characterized as adhesion molecules located on the cell surface. Integrins are a family of cell surface glycoprotein receptors that are involved in both intercellular and extracellular molecule binding, thereby allowing the organization of cells and tissues. From invertebrates to vertebrates, integrins and cadherins have been shown to play an important role in fertilization, embryo development and placentation. Specifically in mammals, ECM interactions with the epithelium hosting each embryonic stage play a critical role in the fate of the developing embryo, its ability to migrate to the uterus, to implant and grow until birth. This study presents new data on the involvement of adhesion molecules in the reproductive process. In order to investigate the role of cellular interactions with extracellular space molecules in the reproductive process, we studied the effect of the ovarian stimulation protocol and embryo biopsy on the expression of fetal integrins and cadherins, as well as their possible regulation of expression in the presence of L-carnitine (L-Cn). We focused on the expression of the integrins avb3, α5b1, av, α6b1 and E-cadherin in zygotes and different stages of embryos grown in vitro or isolated directly from the animal in vivo. Only the in vivo developing embryos showed a specific and polarized distribution of these molecules, indicating their possible successful interaction with the endometrium. The results presented in this study showed that an embryo that is considered to be healthy, according to the morphological criteria by microscopic observation, may be unsuitable for implantation due to the abnormal expression of adhesion molecules. The observation that fetal biopsy reduces the expression of adhesion molecules, most likely due to the invasive nature of the technique, could explain the failure of morphologically and genetically healthy embryos to implant. The addition of L-Cn to the embryonic culture medium, as a powerful energy generating factor, has been shown to enhance the expression of adhesion molecules in pre-implantation embryos, simulating thus the in vivo phenotype of adhesion molecules in the developing embryos, which is important for the acquisition of early embryo polarisation. Early polarity determines the subsequent development of the embryo and its successful implantation. Within the organism, such polarity is dictated by the tubal epithelial cells through interactions with the adhesion molecules, which is not observed in the in vitro developing embryos. Further development of the technology could provide biomimetic surfaces resembling the tubal environment, thereby providing a natural developmental environment for the embryos, and thus an improved embryo implantation outcome.
Language Greek
Subject Embryo biopsy
In vitro fertilizazione
Απεικόνιση THG
Βιοψία εμβρύου
Διέγερση ωοθηκών
Εξωσωματική γονιμοποίηση
Καλλιέργεια προεμφυρτευτικών εμβρύων
Μόρια συνάφειας
Issue date 2020-12-17
Collection   School/Department--School of Medicine--Department of Medicine--Doctoral theses
  Type of Work--Doctoral theses
Permanent Link Bookmark and Share
Views 29

Digital Documents
No preview available

No permission to view document.
It won't be available until: 2023-12-17