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Identifier

000446296

Title

Τα miRNAs ως προγνωστικοί και προβλεπτικοί δείκτες στην Ακτινοθεραπεία του πρώιμου καρκίνου του προστάτη

Alternative Title

Mirnas as prognostic and predictive markers in radiotherapy of early prostate cancer

Author

Κάχρης, Στέφανος

Thesis advisor

Μαυρουδής, Δημήτριος

Reviewer

Σπαντίδος, Δημήτριος
Σοφράς, Φραγκίσκος

Abstract

Radiotherapy is a major treatment option for patients with localized prostate cancer. There are no suitable biomarkers to predict treatment outcome or to reveal a high-risk group of patients for disease progression, so that appropriate treatment decisions can be made in a timely manner. In the present study we aimed to investigate the significance of miRNAs in the outcomes of prostate cancer patients undergoing radiotherapy and to identify the related pathways through bioinformatics analysis. The expression levels of miR- 21, miR-106b, miR-141 and miR-375 involved in the response to radiotherapy were assessed by RT-qPCR in the serum of PCa patients (n=56) prior- and post- RT. Low expression levels of miR-106b prior-RT were associated with extracapsular extension and seminal vesicles invasion by the tumor (p=0.031 and 0.044, respectively). In the high-risk subgroup (n=47), post-RT expression levels of miR- 21 were higher in patients with biochemical relapse (BR) compared to non-relapse (p=0.043). Also, in the salvage treatment subgroup (post-operative BR; n=20), post-RT expression levels of miR-21 and miR-106b were higher in patients with BR compared to non-relapse (p=0.043 and p=0.032, respectively). In the whole group of patients, high expression levels of miR-21 prior-RT and of miR-106b post-RT were associated with significantly shorter overall survival (OS; p=0.049 and p=0.050, respectively). No associations were observed among miR- 141 and miR-375 expression levels with clinicopathological features or treatment outcome. Bioinformatics analysis revealed significant enrichment in DNA damage response pathways. In conclusion, circulating miRNAs prior or post-RT may hold prognostic implications in patients with PCa.

Language

Greek

Subject

Circulating mirnas

Real-time PCR

Κυκλοφορούντα mirnas

Issue date

2022-03-30

Collection

School/Department--School of Medicine--Department of Medicine--Doctoral theses

Type of Work--Doctoral theses

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