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Identifier

uch.med.phd//1996DIS0077

Title

Διερεύνηση των μηχανισμών της μετα-μεταγραφικής ρύθμισης του πρωτο-ογκονιδίου C-MYC

Alternative Title

Study of the post-transcriptional regulation of the C-MYC proto-oncogene

Creator

Ioannidis, Panagiotis G

Abstract

Our goal was to elucidate the IFN-a induced alterations on the degradation process of c-myc mRNA.Intermediate degradation products were detected in trasfected cells overexpressing structurally identical to normal c-myc mRNA.It was demonstrated that c-myc mRNA degradation follows two pathways.Rapid deadenylation results in the accumulation of oligoadenylated c-myc mRNA due to a endonucleolytic cleavage without prior deadenylation.Exposure to INF-a enhances this mechanism. Inhibition of protein synthesis does not prevent the formation of products of endonucleolytic cleavage.The traslability of the oligoadenylated c-myc mRNA generated in vivo,in the presence of cordycepin (3'dA) was determined.Our data indicate that oligoadenylated c-myc mRNA synthesized in the presence of cordicepin is more efficient as a template for protein synthesis compared to its counterart with heterogeneous distribution of poly(A) tails c-myc mRNA of control cells. The data suggest that the naturally generated oligoadenyl c-nyc mRNA through the deadadenylation process possibly represents the translationally active form of c-myc mRNA.

Issue date

1996-07-01

Date available

1997-06-6

Collection

School/Department--School of Medicine--Department of Medicine--Doctoral theses

Type of Work--Doctoral theses

Permanent Link

https://elocus.lib.uoc.gr//dlib/e/a/0/metadata-dlib-1996DIS0077.tkl

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