Doctoral theses
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Current Record: 997 of 1068
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Identifier |
uch.med.phd//1996DIS0077 |
Title |
Διερεύνηση των μηχανισμών της μετα-μεταγραφικής ρύθμισης του πρωτο-ογκονιδίου C-MYC |
Alternative Title |
Study of the post-transcriptional regulation of the C-MYC proto-oncogene |
Creator |
Ioannidis, Panagiotis G
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Abstract |
Our goal was to elucidate the IFN-a induced alterations on the degradation process of c-myc mRNA.Intermediate degradation products were detected in trasfected cells overexpressing structurally identical to normal c-myc mRNA.It was demonstrated that c-myc mRNA degradation follows two pathways.Rapid deadenylation results in the accumulation of oligoadenylated c-myc mRNA due to a endonucleolytic cleavage without prior deadenylation.Exposure to INF-a enhances this mechanism. Inhibition of protein synthesis does not prevent the formation of products of endonucleolytic cleavage.The traslability of the oligoadenylated c-myc mRNA generated in vivo,in the presence of cordycepin (3'dA) was determined.Our data indicate that oligoadenylated c-myc mRNA synthesized in the presence of cordicepin is more efficient as a template for protein synthesis compared to its counterart with heterogeneous distribution of poly(A) tails c-myc mRNA of control cells. The data suggest that the naturally generated oligoadenyl c-nyc mRNA through the deadadenylation process possibly represents the translationally active form of c-myc mRNA.
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Issue date |
1996-07-01 |
Date available |
1997-06-6 |
Collection
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School/Department--School of Medicine--Department of Medicine--Doctoral theses
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Type of Work--Doctoral theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/e/a/0/metadata-dlib-1996DIS0077.tkl
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Views |
275 |