Graduate theses
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Current Record: 4 of 1665
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Title |
Αποικοδόμηση πολυδροξυλικών αρωματικών ενώσεων από το βακτήριο Pseudomonas sp.phDV1 |
Alternative Title |
Degradation of polydroxy aromatic compounds by the bacterium Pseudomonas sp.phDV1 |
Author
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Γκίκας, Γεώργιος
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Thesis advisor
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Τσιώτης, Γεώργιος
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Abstract |
One of the burning issues of the modern world is the issue of plastics and the pollution of
the environment by these materials. Plastics, due to the fact that they are nonbiodegradable and remain almost intact over time, are one of the most significant threats
to terrestrial and aquatic ecosystems. For this reason, more and more companies are
trying to manufacture new materials, such as bioplastics, which will replace plastics and
will be much more ecological and environmentally friendly. Poly-hydroxyalkanoates (PHAs)
are a class of polymers, which are produced by microorganisms and through them
bioplastics are made, which have excellent biodegradability. In this work, the production of
PHB, which is the most widely used and studied poly-hydroxyalkanoic acid, by the
bacterial strain Pseudomonas sp. phDV1 is studied. More specifically, this aerobic and
Gram-negative bacterial strain has the ability to produce PHB pellets (or carbosomes)
inside itself when under stress. To produce these carbosomes, therefore, the strain was
grown in different carbon sources such as phenol and other monocyclic polyhydroxy
aromatic compounds, which the bacterium uses as a carbon source. PHB was quantified
by measuring the amount of crotonic acid by HPLC and observation of the bacterial
carbosomes was carried out by optical microscope. The metabolism of aromatic
compounds by the bacterium was studied by visible spectroscopy and through NMR 1H
technique.
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Language |
Greek |
Subject |
Carbosomes |
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HPLC |
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NMR 1H |
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Poly-hydroxyalkanoic acids (PHAs) |
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Polyhydroxybutyric acid (PHB) |
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Καρβοσώματα |
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Πολυ-υδροξυαλκανοϊκά οξέα (PHAs) |
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Πολυυδροξυβουτυρικό οξύ (PHB) |
Issue date |
2024-07-19 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
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Type of Work--Graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/a/6/2/metadata-dlib-1719904905-483112-10896.tkl
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Views |
1527 |