Graduate theses
Current Record: 42 of 1665
|
Title |
Έλεγχος ποιότητας σε μελέτες μεταβολομικής με υγρή χρωματογραφία συζευγμένη με χρωματογραφία μάζας. Μελέτη απόδοσης αλγορίθμων επεξεργασίας δεδομένων. |
Author
|
Καραπιδάκη, Ανθή
|
Thesis advisor
|
Χατζηιωάννου, Αναστασία-Χρυσοβαλάντου
|
Abstract |
In the modern period, emphasis has turned toward holistic analysis and maximizing
the collected information from each sample (sample's "fingerprint"). The term
metabolomics refers to the comprehensive study of all metabolites in a given biological
sample. Metabolomic analysis typically results in large datasets therefore, and the
extraction of meaningful information out of these data sets is a significant part of the
workflow.
The primary goal of this thesis was to utilize and assess the capabilities of an online
tool for the preprocessing and analysis of unprocessed metabolomic data, namely
MetaboAnalyst. Additionally, we compared the results of the MetaboAnalyst analysis
with those obtained from the analysis using a commercially available software from
AGILENT, which has been extensively used in such analyses. The samples processed
in this work are quality control (QC) samples and blank samples, which were analyzed
using the LC-HRMS method in a large-scale study lasting multiple weeks.
In conclusion, the results obtained from the various algorithms within
MetaboAnalyst, as well as the analysis conducted in MetaboAnalyst and Agilent
software exhibit significant differences. Consequently, the inference can be drawn that
the preprocessing of raw data in metabolomic studies is a crucial and integral component
of the analysis. Varied choices in this particular aspect of the analysis can lead to entirely
different outcomes, thereby influencing dramatically the subsequent statistical analyses.
|
Language |
Greek |
Issue date |
2024-03-21 |
Collection
|
School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
|
|
Type of Work--Graduate theses
|
Permanent Link |
https://elocus.lib.uoc.gr//dlib/c/0/c/metadata-dlib-1710157098-563596-5045.tkl
|
Views |
334 |