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Title Secretome Analysis of Lymphoma cells after reactivation of wild type p53
Author Πιτσικάκη, Ανθή
Thesis advisor Παυλίδης, Ιωάννης
Αϊβαλιώτης, Μιχαήλ
Abstract The aim of this diploma thesis is the comparative study of the proteins secreted by human lymphoma cell lines, before and after the reactivation of wt-p53 with Nutlin-3a. Nutlin-3a is a small molecule that acts as an antagonist of the p53-MDM2 complex. In particular, after the collection of the protein secretome of the cultured cells in serum-free medium, techniques such as separation of the proteins by electrophoresis into a polyacrylamide gel or gel free methods take place. These techniques are followed by the enzymatic digestion of the proteins with a selective protease and their identification and quantification by high sensitivity analysis with tandem mass spectrometry coupled with nano-liquid chromatography (nanoLC - MS/MS). Final step is the investigation of their interactions and their possible involvement in the prognosis, diagnosis and treatment of lymphomagenesis, followed by bioinformatics analysis.
Language English
Issue date 2019-07-24
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Chemistry--Graduate theses
  Type of Work--Graduate theses
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