Your browser does not support JavaScript!

Home    Collections    Type of Work    Graduate theses  

Graduate theses

Current Record: 29 of 1622

Back to Results Previous page
Next page
Add to Basket
[Add to Basket]
Title The use of optical projection tomography technique for tomographic imaging of mice skulls
Alternative Title Η χρήση της τεχνικής Optical Projection Tomography για την τομογραφική απεικόνιση κρανίων ποντικιών
Author Σταυρουλάκης, Σπυρίδων
Thesis advisor Ζαχαράκης, Ιωάννης
Abstract Now at a time when we can use relatively new imaging techniques to study whole rodent organs as small as a few centimeters. Such a method is mesoscopy. An important technique in this field is optical projection tomography (OPT). In this paper we review the use of the OPT technique, assisted by fluorescence, on biological specimens to study craniosynostosis disease. These biological samples consist of processed mice skulls. We analyze the principles of the OPT method, focusing on the filtered-back projection algorithm, which is the basic tomographic method for object reconstruction. We apply the use of optical projection tomography for full 3D reconstruction of 6 prepared skulls. Initially, the skull is reconstructed using OPT, with the help of LED illumination, calculating the light intensity before and after the light is absorbed by the specimen. Due to limitations faced in terms of analytical study of skull anatomy, we added the concept of fluorescence microscopy, where the regions of high interest in the specimen are also the fluorescent markers. The instruments and experimental setup we used in the experiments are presented, as well as the preparation of each specimen before the acquisition of the images. As an extension of the preceding, the importance of the number of projections on the quality of the reconstructed image is explained. Although we encountered some difficulties in isolating the spectra of the two fluorescent markers using filters alone, we examined the value of the Linear Unmixing method for separating fluorescent signals and quantifying cartilage and bone relative to the whole mouse skull. Finally, considering the results of the experiments, we study the shape of the cartilage that separates the bones of the skull from each other and thus our conclusions about craniosynostosis disease are drawn.
Language English
Issue date 2023-11-22
Collection   School/Department--School of Sciences and Engineering--Department of Physics--Graduate theses
  Type of Work--Graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/7/4/8/metadata-dlib-1698126818-896858-15880.tkl Bookmark and Share
Views 201

Digital Documents
No preview available

Download document
View document
Views : 1