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Title Ο ρόλος της πρωτεΐνης PML στη ρύθμιση της πλειοδυναμίας βλαστοκυττάρων μέσω σουμοϋλίωσης πρωτεϊνών
Alternative Title The role of PML protein in the regulation of stem cell pluripotency via protein sumoylation
Author Μπαρτζιώκα, Στέλλα
Thesis advisor Σπηλιανάκης, Χαράλαμπος
Κρετσόβαλη, Ανδρονίκη
Abstract Promyelotic Leukemia protein (PML) is a tumor suppressor protein that is involved in several processes. The PML protein can sumoylate other target proteins through the nuclear bodies it forms. Previous proteomic analysis of sumoylated proteins from embryonic stem cells, after deletion of the Pml gene, showed that proteins that regulate embryonic stem cell pluripotency, such as Spalt Homology 1 (SALL1) protein, are significantly undersumoylated. The SALL1 protein has two roles in embryonic stem cells – it is both a transcriptional repressor and a transcriptional co-activator. The aim of this thesis is to study the importance of sumoylation in the functions of the SALL1 protein. In particular, the interactions of SALL1 protein with other pluripotency factors and the stability of SALL1 protein were studied. For this reason, a plasmid containing the cDNA of the SALL1 protein was constructed. Using the single-primer site-directed mutagenesis method, the codons that corresponded to lysine amino acid residues, which are usually sumoylated, were mutated. Co-immunoprecipitation experiments were conducted to observe the interactions of the non-sumoylated GFP-SALL1 protein with SOX2 and NANOG proteins. The stability of the SALL1 protein was studied by protein synthesis control experiments. The conclusions were that sumoylation of SALL1 protein does not regulate its interaction with SOX2 protein, whereas sumoylation negatively regulates its interaction with NANOG protein. Furthermore, it was observed that sumoylation contributes to the stability of SALL1 protein. Understanding SALL1 sumoylation is significant, as it may provide insights into how SALL1 protein contributes to the maintenance of embryonic stem cell pluripotency and thus how it can be exploited in the field of regenerative medicine.
Language Greek
Subject SALL1
Εmbryonic stem cells
Εμβρυϊκά βλαστοκύτταρα
Issue date 2023-11-24
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Graduate theses
  Type of Work--Graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/b/8/4/metadata-dlib-user1700067668-27752.tkl Bookmark and Share
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