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Title Μελέτη της παραγωγής βιοϋδρογόνου από ένα αζωτοδεσμευτικό βακτήριο σε διαφορετικά υποστρώματα σακχάρων
Alternative Title A study of biohydrogen production by a nitrogen-fixing bacterium under different carbon substrates
Author Τσιχλάκη, Αλεξάνδρα Αλεξία
Thesis advisor Γανωτάκης, Δημήτριος
Abstract In this thesis, the effect of various growth conditions on the cell growth and hydrogen production by the nitrogen-fixing bacterium Clostridium pasterianum was investigated. This bacterium has the ability to produce biohydrogen through the process of dark fermentation, which is an environmentally friendly fuel that can help reduce environmental pollution. Initially, the effect of the inoculum size was studied, as well as the growth of the microorganism in nutrient media with different carbon and nitrogen substrates. The above parameters had a significant effect on hydrogen production and cell growth. Next, the growth and hydrogen production were studied under different sugar substrates, as well as their concentrations. Cultures of the bacterium grown on mannitol, sorbitol, glucose and fructose showed the highest hydrogen production, even at the lowest sugar concentrations, while on disaccharides such as maltose and sucrose, the production was comparatively lower. In addition, hydrolyzed cheese whey with high organic load was used as a growth medium for the bacterium. According to the results, the microorganism showed both cell growth and hydrogen production at close levels to the control cultures. From the results of this study, it was concluded that the rational design of growth parameters can optimize biohydrogen production during the dark fermentation by the bacterium. Furthermore, the use of industrial wastewaters, such as cheese whey, as a growth media allows the sustainable production of biofuels with lower economic costs
Language Greek
Subject Carbon source
Clostridium pasteurianum
Dairy wastewaters
Dark fermentation
Growth
Hydrogen
Nitrogen-fixing bacteria
Αζωτοδεσμευτικά βακτήρια
Ανάπτυξη
Απόβλητα τυροκομείου
Πηγή άνθρακα
Σκοτεινή ζύμωση
Υδρογόνο
Issue date 2024-03-21
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
  Type of Work--Graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/2/e/5/metadata-dlib-user1710172548-12813.tkl Bookmark and Share
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