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Title Επεξεργασία στεμφύλων μέσω συνδυασμού μικροοργανισμών, με σκοπό την αποδόμηση πολυφαινολών και την ασφαλή απόρριψή τους στο περιβάλλον
Author Χατζάκης, Εμμανουήλ
Thesis advisor Γανωτάκης, Δημήτριος
Abstract Grape pomace constitutesthe main by-product of vinification and comprisesthe pulp of grape skins, seeds and stems. Grape pomace contains a wide variety of organic compounds, including polyphenols which can be found in large amounts. Their presence in pomace renders its disposal quite hazardous, due to the toxic effects they might instigate. The present study focuses on the qualitative analysis of polyphenols in grape pomace and the treatment with microorganisms, in order to reduce their levels. The research was conducted on pomace from the grape variety of ‘Kotsifali’, a native variety cultivated in Crete. The pomace was dried and later pulverised in order to obtain a proper form for secondary processing. Subsequently, microbial cultures were created with the pomace, utilizing the commercial products Uniker, Multicraft and Microferm, which contain a variety of microorgasims. In addition, experiments were carried out with Chlorella, a photosynthetic microorganism, and Saccharomyces cerevisiae. The samples obtained from the cultures were analyzed with HPLCHigh Performance Liquid Chromatography, for the exact monitoring of polyphenol levels. The purpose was to ascertain, whether the microorganisms could potentially biodegrade polyphenols, lead to the reduction of their levels in pomace and thus contribute to pomace’s bioremediation and safe disposal.
Language Greek
Issue date 2021-11-24
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
  Type of Work--Graduate theses
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