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Title

Αποικοδόμηση πολυδροξυλικών αρωματικών ενώσεων από το βακτήριο Pseudomonas sp.phDV1

Alternative Title

Degradation of polydroxy aromatic compounds by the bacterium Pseudomonas sp.phDV1

Author

Γκίκας, Γεώργιος

Thesis advisor

Τσιώτης, Γεώργιος

Abstract

One of the burning issues of the modern world is the issue of plastics and the pollution of the environment by these materials. Plastics, due to the fact that they are nonbiodegradable and remain almost intact over time, are one of the most significant threats to terrestrial and aquatic ecosystems. For this reason, more and more companies are trying to manufacture new materials, such as bioplastics, which will replace plastics and will be much more ecological and environmentally friendly. Poly-hydroxyalkanoates (PHAs) are a class of polymers, which are produced by microorganisms and through them bioplastics are made, which have excellent biodegradability. In this work, the production of PHB, which is the most widely used and studied poly-hydroxyalkanoic acid, by the bacterial strain Pseudomonas sp. phDV1 is studied. More specifically, this aerobic and Gram-negative bacterial strain has the ability to produce PHB pellets (or carbosomes) inside itself when under stress. To produce these carbosomes, therefore, the strain was grown in different carbon sources such as phenol and other monocyclic polyhydroxy aromatic compounds, which the bacterium uses as a carbon source. PHB was quantified by measuring the amount of crotonic acid by HPLC and observation of the bacterial carbosomes was carried out by optical microscope. The metabolism of aromatic compounds by the bacterium was studied by visible spectroscopy and through NMR ¹H technique.

Language

Greek

Subject

Carbosomes

HPLC

NMR 1H

Poly-hydroxyalkanoic acids (PHAs)

Polyhydroxybutyric acid (PHB)

Καρβοσώματα

Πολυ-υδροξυαλκανοϊκά οξέα (PHAs)

Πολυυδροξυβουτυρικό οξύ (PHB)

Issue date