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Title Understanding the optical response of anisotropic scattering media and their benefits to Light Sheet Fluorescence Microscopy
Alternative Title Κατανοώντας την οπτική ανταπόκριση των ανισοτροπικών σκεδαστικών μέσων και τα οφέλη τους στην Μικροσκοπία Φθορισμού Φύλλου Φωτός
Author Μαννέ, Ηρώ
Thesis advisor Ζαχαράκης, Ιωάννης
Abstract This study investigates the optical response of a novel anisotropic medium and its advantages in Light Sheet Fluorescent Microscopy (LSFM). LSFM, renowned for its high resolution and multi-dimensional imaging, is based on a single plane illumination of the sample using a laser beam shaped into a light sheet. This investigation aims to provide deeper insights into the properties of this newly developed medium and its potential applications in enhancing LSFM imaging quality. The study encompasses an exploration of light propagation in a medium, providing the theoretical background in scattering, and the Spatial Light Modulator operation in wavefront shaping. Experimentally, the research involves fabricating the anisotropic scattering medium, the goniophotometer analysis of its angular intensity. Additionally, an analysis of its surface morphology from SEM images was performed, as well as a reproducibility evaluation of the fabrication process. Results demonstrate a high correlation in the medium’s behavior with a typical diffuser, due to the dispersed energy distribution across one direction. The SEM analysis highlights the statistical properties of its surface which can be used in future optical simulations and the reproducibility assessment showcases a general trend towards similar values of the light sheet.
Language English
Issue date 2024-07-11
Collection   School/Department--School of Sciences and Engineering--Department of Physics--Graduate theses
  Type of Work--Graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/9/9/8/metadata-dlib-1719296765-357042-11090.tkl Bookmark and Share
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