Graduate theses
Current Record: 29 of 1623
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Title |
The use of optical projection tomography technique for tomographic imaging of mice skulls |
Alternative Title |
Η χρήση της τεχνικής Optical Projection Tomography για την τομογραφική απεικόνιση κρανίων ποντικιών |
Author
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Σταυρουλάκης, Σπυρίδων
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Thesis advisor
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Ζαχαράκης, Ιωάννης
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Abstract |
Now at a time when we can use relatively new imaging techniques to study whole rodent
organs as small as a few centimeters. Such a method is mesoscopy. An important technique in
this field is optical projection tomography (OPT). In this paper we review the use of the OPT
technique, assisted by fluorescence, on biological specimens to study craniosynostosis disease.
These biological samples consist of processed mice skulls. We analyze the principles of the OPT
method, focusing on the filtered-back projection algorithm, which is the basic tomographic
method for object reconstruction. We apply the use of optical projection tomography for full
3D reconstruction of 6 prepared skulls. Initially, the skull is reconstructed using OPT, with the
help of LED illumination, calculating the light intensity before and after the light is absorbed by
the specimen. Due to limitations faced in terms of analytical study of skull anatomy, we added
the concept of fluorescence microscopy, where the regions of high interest in the specimen are
also the fluorescent markers. The instruments and experimental setup we used in the
experiments are presented, as well as the preparation of each specimen before the acquisition
of the images. As an extension of the preceding, the importance of the number of projections
on the quality of the reconstructed image is explained. Although we encountered some
difficulties in isolating the spectra of the two fluorescent markers using filters alone, we
examined the value of the Linear Unmixing method for separating fluorescent signals and
quantifying cartilage and bone relative to the whole mouse skull. Finally, considering the results
of the experiments, we study the shape of the cartilage that separates the bones of the skull
from each other and thus our conclusions about craniosynostosis disease are drawn.
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Language |
English |
Issue date |
2023-11-22 |
Collection
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School/Department--School of Sciences and Engineering--Department of Physics--Graduate theses
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Type of Work--Graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/7/4/8/metadata-dlib-1698126818-896858-15880.tkl
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Views |
214 |