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Title

In vivo απεικόνιση της ανάπτυξης εμβρύων Parhyale hawaiensis με την χρήση υβριδικής confocal και φωτοακουστικής μικροσκοπίας

Alternative Title

In vivo imaging of the development of Parhyale hawaiensis using confocal and photoacoustic microscopy

Author

Καζάση, Ευτυχία

Thesis advisor

Παυλίδης, Μιχαήλ

Abstract

The amphipod Parhyale hawaiensis, belonging to the broader category of crustaceans, serves as an emerging model organism for developmental, genetic, and evolutionary analyses. Recently, a plethora of optical microscopy techniques, primarily fluorescence-based, have been employed to study Parhyale regarding its anatomical features, patterns of gene expression, and reconstruction of cell lineages. Aiming to gather additional information, a proposal is made for a new low-cost hybrid imaging system that combines features of fluorescence and frequency-domain photoacoustic microscopy, simultaneously recording both radiative and radiationless molecular relaxations after the sample excitation from an intensity-modulated laser source. The hybrid microscope is evaluated for its ability to provide in vivo imaging of wild-type unlabeled and fluorescently labeled Parhyale embryos at different developmental stages. The two combined imaging techniques offer highly complementary information regarding cell positioning, yolk distribution within them, nucleus movement, cell membranes, and the behavior of various cell structures during embryogenesis. Findings from this study will aid in a better understanding of the emerging model organism Parhyale hawaiensis and can serve as a starting point for the wider adoption of affordable hybrid optical and photoacoustic microscopy methods in developmental biology, thus significantly enhancing the capabilities of the technologies used today.

Language

Greek

Subject

Developmental biology

Embryogenesis

Fluorescence

Hybrid imaging

Label-free

Yolk

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