Post-graduate theses
Current Record: 34 of 6713
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Identifier |
000466103 |
Title |
Διαλεύκανση του ρόλου των HSP90 στο μονοπάτι μεταγωγής σήματος των Βρασσινοστεροειδών |
Alternative Title |
Elucidation of the role of HSP90 in Brassinosteroids signaling pathway |
Author
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Γκριτζάς, Ραφαήλ Α
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Thesis advisor
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Χατζόπουλος, Πολυδεύκης
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Reviewer
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Μηλιώνη, Δήμητρα
Καλαντίδης, Κρίτων
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Abstract |
The HSP90 (HEAT SHOCK PROTEIN 90) play an active role in fine-tuning physiological responses and
developmental processes in the plant Arabidopsis thaliana. The multifaceted function of HSP90 is achieved
through a wide range of molecular networks in which they are involved, interacting, dissociating, and
connecting. A notable example is the Brassinosteroid pathway, where HSP90 naturally interacts with members
of the heterodimeric receptor BRI1/BAK1, the kinase BIN2, and the transcription factors BES1/BZR1. The
impact of HSP90 on maintaining the membrane and intracellular levels of BRI1 suggests their involvement in
receptor’s recycling mechanism. This is supported by data indicating a similar effect on the auxin transporter
PIN1. In the context of this thesis, observations were made using fluorescence confocal microscopy
techniques, focusing on the differential subcellular localization and changes in membrane and overall levels
of the BRI1 receptor in the Arabidopsis root after pharmacological or genetic reduction of HSP90 activity.
Simultaneously, efforts were made to identify the points of BRI1 receptor recycling pathway involving HSP90
through comparative phenotypic analysis using drugs that block recycling (Concanamycin A and
Wortmannin) at specific sites. In addition, the possibility of interaction between cytoplasmic HSP90 and the
demethylation of histones H3K27, RELATIVE OF EARLY FLOWERING 6 (REF6), was investigated,
which, among other things, is involved in the expression of brassinosteroid-regulated genes. Summarizing all
results, HSP90 plays a central role in fine-tuning the brassinosteroid signal transduction pathway by engaging
in the recycling pathways of BRI1 and controlling the epigenetic status of brassinosteroid-responsive genes,
interacting with REF6.
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Language |
Greek |
Subject |
Brassinosteroid receptor |
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Early endosomes |
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Endocytosis |
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Heat shock proteins |
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Late endosomes |
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Lytic vacuoles |
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Pharmacological inhibition |
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Plasma membrane |
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Recycling |
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Ώριμα ενδοσώματα |
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Ανακύκλωση |
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Ενδοκύττωση |
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Θερμοεπαγώμενες πρωτεΐνες |
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Λυτικά χυμοτόπια |
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Πλασματική μεμβράνη |
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Πρώιμα ενδοσώματα |
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Υποδοχέας Βρασσινοστεροειδών |
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Φαρμακευτική αναστολή |
Issue date |
2024-07-26 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/d/c/4/metadata-dlib-1721036531-127841-402.tkl
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Views |
664 |