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Identifier

000420012

Title

LKB1 loss in colorectal cancer patients’ samples

Author

Alam, Sardar

Thesis advisor

Sougklakos, Ioannis

Abstract

Lkb1 is considered as a tumor suppressor gene in Puetz-Jegeher syndrome (PJS) as well as in sporadic cancer. Its inactivation due to loss of heterozygosity (LOH) has been reported in some PJS patients, ovarian as well as in lung cancer. We aimed to identify loss of LOH in a relatively large number of colon carcinomas (n=207). We used two different techniques to identify LOH in Lkb1. We identified LOH in 14% colon cancer cases by IHC only. Lkb1 simultaneous nuclear and cytoplasmic (catalytic mutants Lkb1) expression was more common in the metastatic group than the non-metastatic group. We observed two cases having exclusively nuclear staining, and both were from metastatic patients. Our results suggest that LKb1 has a pro-oncogenic role in colon cancer as opposed to its tumor suppressor role because in the metastatic group we observed a higher number of patients that showed positive LKB1 expression than the non- metastatic group. Also, there is a weak positive correlation between normal colon and colon carcinomas on the basis of their Lkb1 expression. We did not detect LOH in 120 samples using microsatellite assay by utilizing 4 different microsatellite markers for the LKB1 locus. We used gene mapping for 120 samples only, in the remaining cases (n=87), we don’t have good quality samples.

Language

English

Subject

Immunohistochemistry

LKB1 Colorectal cancer

Issue date

2018-12-05

Collection

School/Department--School of Medicine--Department of Medicine--Post-graduate theses

Type of Work--Post-graduate theses

Permanent Link

https://elocus.lib.uoc.gr//dlib/1/7/3/metadata-dlib-1544691658-709882-19762.tkl