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Identifier 000433890
Title Extraction of mouse brain extracellular matrix by decellularization for porous scaffold fabrication
Alternative Title Απομόνωση εξωκυττάριας μήτρας εγκεφάλου ποντικού μέσω αποκυτταροποίησης για δημιουργία πορώδους ικριώματος
Author Μαραυγάκη, Ερασμία Ιωάννα
Thesis advisor Γραβάνης, Αχιλλέας
Χαραλαμπόπουλος, Ιωάννης
Τζεράνης, Δημήτριος
Abstract The extracellular matrix (ECM) is a complex of proteins and proteoglycans that contributes to normal physiology of tissues. The ECM of the Central Nervous System (CNS) is important for multiple processes, including basal lamina formation, neural communication and synaptic plasticity stabilization. Alterations to ECM due to injury (e.g. spinal cord injury, stroke) or disease (e.g. Alzheimer’s disease) can have dramatic consequences. Since ECM can simulate cells, acellular biologic scaffolds are used for tissue remodeling after damage and have promising applications in CNS regenerative medicine. In this master thesis a new decellularized process was developed in order to purify decellularized brain ECM from adult mouse brain tissue. Decellularized tissue was broken in pieces and lyophilized in order to produce ECM powder, which is a raw material appropriate for scaffold fabrication. The obtained decellularized brain ECM was characterized for its bio-compability and toxicity and demonstrated neuroprotective actions when used as a medium supplement in PC12 and NSC34 cell lines in vitro. Furthermore, the obtained decellularized brain ECM was able to regulate the adhesion of primary Embryonic Cortical Neural Stem Primary Cells (NSCs) without affecting their survival or differentiation. These results suggest that the obtained decellularized brain ECM provides neuro-protective ECM components, which can be used for fabricating porous scaffolds and potentially advance NSC-based grafts for CNS regenerative medicine applications.
Language English
Subject Αποκυτταροποίηση
Εξωκυττάρια μήτρα
Πορώδες ικρίωμα
Issue date 2020-12-17
Collection   School/Department--School of Medicine--Department of Medicine--Post-graduate theses
  Type of Work--Post-graduate theses
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