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Identifier 000452367
Title Quantifying cell-matrix interactions in laser microfabricated scaffolds
Alternative Title Ποσοτικοποίηση αλληλεπιδράσεων κυττάρων-μήτρας σε μικροκατεργασμένα με λέϊζερ ικριώματα
Author Μοσχογιαννάκη, Φερενίκη
Thesis advisor Στρατάκης, Εμμανουήλ
Reviewer Τζεράνης, Δημήτριος
Χαραλαμπόπουλος, Ιωάννης
Abstract This thesis studies how cell phenotypes of interest can be regulated by the physicochemical properties of their surrounding insoluble microenvironment (matrix). Specifically, it studies how the microfabrication of specific μm-sized patterns in porous collagen-based scaffolds (PCS) can regulate key phenotypes of fibroblasts and Neural Stem Cells, two cell types involved in wound healing. By using Fs laser ablation, three different patterns were microfabricated on the scaffolds. In addition to the ‘’plain’’ control PCS, ablation enabled the fabrication of a PCS with parallel horizontal lines, a PCS with concentric circles and a PCS that contained a hole in its center. The first experiment monitored the contractile response of fibroblasts when seeded in PCS that contained various microfabricated patterns. Live cell imaging revealed significant PCS deformation due to fibroblast contraction. Immunolabeling for α smooth muscle actin (α-SMA) in the PCS that contained concentric cycles revealed the presence of α-SMA+ cells (myofibroblasts) on the outer surface of circular features. The second experiment studied whether differentiation of Neural Stem Cells towards neurons and astrocytes depend on the presence and type of microfabricated features. Immunolabeling for Tuj1 (neurons) and GFAP (astrocytes) revealed that PCS with concentric circles decreased the fraction of cells that differentiated towards neurons and astrocytes. This thesis provides a first proof of concept that microfabricated features in PCS can regulate cell phenotypes, although it did not study in detail its underlying molecular mechanisms.
Language English, Greek
Subject Collagen
Κολλαγόνο
Μικροκατεργασία
Τεχνική μηχανικής εκμάθησης
Issue date 2022-12-07
Collection   School/Department--School of Medicine--Department of Medicine--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/9/e/9/metadata-dlib-1669372960-494394-26126.tkl Bookmark and Share
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