Post-graduate theses
Current Record: 2 of 6513
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| Identifier |
000463817 |
| Title |
Synthesis and characterization of bioinks for bioprinting |
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Author
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Πετρίδη, Ηλιάνα
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Thesis advisor
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Χατζηνικολαϊδου, Μαρία
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Reviewer
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Andersen, Peter E.
Untracht, gavrielle R.
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| Abstract |
Tissue engineering constitutes a developing field that aims to create structures to replace damaged and/ or diseased tissue components, ensuring the well-being of the people experiencing health issues because of the problems induced. For this purpose, the use of biomaterials and cells is a main factor contributing to the field of tissue engineering. To establish that these structures are suitable for their aimed application, they need to be fully characterized before moving on to their incorporation in clinical practice. In this thesis, I studied bioinks, 3D bioprinted hydrogels embedded with cells with the aim of forming structures similar to vessels, with regard to their optical properties, in an effort to study how they behave when interacting with light, using an imaging modality called Optical Coherence Tomography (OCT). The bioinks were developed using laponite, a synthetic clay, and gellan gum, a bacterial polysaccharide for their matrix and they were loaded with mesenchymal stem cells. Four different sets of samples were studied, with varying composition of laponite, namely 0.5% and 1%. Five different time-points per sample set were examined, to study how the degradation process affects the optical properties. These were developed at the University of Crete, in collaboration with PhD candidate Varvara Platania and were imaged at the Technical University of Denmark. For the imaging part, Spectral Domain Optical Coherence Tomography was employed with a central wavelength of 1310 nm, exploiting the technique’s non-destructive character, while maintaining an axial and lateral resolution in the microscale. Post-processing of the images was realized for the extraction of the scattering coefficient implementing a single scattering model, based on the linear fitting of the logarithm of the OCT signal. The results showed that the structure of the sample sets containing 1% laponite were more homogeneous in appearance with better sample surface. Finally, as the degradation progressed I observed that the scattering coefficient values were presenting an advancing trend and this was shown for three of the sample sets studied, while it was determined to disregard the fourth that was subjected to exclusion criteria, due to several observations in its overall behavior
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| Language |
English, Greek |
| Subject |
Laponite |
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Optical coherence tomography |
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Optical properties |
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Scattering |
| Issue date |
2024-04-17 |
| Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
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Type of Work--Post-graduate theses
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| Permanent Link |
https://elocus.lib.uoc.gr//dlib/7/5/4/metadata-dlib-1712641204-160411-10963.tkl
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| Views |
2 |
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