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Identifier 000428732
Title Ανάπτυξη του παθογόνου Coxiella burnetii σε διαφορετικά κύτταρα ξενιστές. Έκφραση υπομονάδων του εκκριτικού συστήματος
Alternative Title Growth of the pathogen Coxiella burnetii in different host cells. Expression of subunits of the secretion system
Author Τρυπάκη, Μαρία Μ.
Thesis advisor Τσιώτης, Γεώργιος
Reviewer Παυλίδης, Ιωάννης
Ψαρουλάκη, Άννα
Abstract The bacterium Coxiella burnetii is the causative agent of Q fever, a zoonotic disease that causes significant health problems and has a significant financial impact on its occurrence. The bacterium is an airborne and highly infectious vector with remarkable resistance to environmental stress, and first described in 1937 in slaughterhouses in Australia. Detailed study of the pathogen has been made difficult due to its obligatory intracellular nature, however, the successful cultivation of Coxiella in different cell lines, the development of an axenic culture medium, and the application of bioinformatics tools, have made significant progress in zoonotic research during this decade. A key factor associated with the pathogenesis of Coxiella is the type IV Dot / Icm secretory system (T4SS), used to transport effector proteins to the host cell cytoplasm. The effector proteins transported through T4SS mediate the survival and successful proliferation of the pathogen within the host cell. In this present work, the Coxiella burnetii NM II bacterium was grown in different hosts (HeLa, VERO and MRC-5 cells) as well as in an axenic culture medium, in order to identify the bacterial and host cell proteins at different days post infection. In addition, expression of the DotC subunit of T4SS in E.coli receptor cells was characterized by mass spectrometry for structural analysis by crystallography.
Language Greek
Subject Dot/Icm system
Dot/Icm σύστημα
Intracellular pathogen
Q fever
Q πυρετός
Ενδοκυτταρικό παθογόνο
Πρωτεωμική ανάλυση
Issue date 2020-03-27
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
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