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Identifier 000360955
Title Improvements of a methodology for functional inactivation of proteases in apicomplexan parasites by using natural or molecular engineered serpins
Alternative Title Βελτιώσεις σε μα μεθοδολογία λειτουργικής απενεργοποίησης πρωτεασών στα ακροσυμπλεγματικά παράσιτα μέσω της χρησιμοποίησης φυσικών ή μοριακά τροποποιημένων σερπινών
Author Μακρογκίκας, Στυλιανός
Thesis advisor Λουκέρης, Θανάσης
Τοκατλίδης, Κώστας
Παπαματθεάκης, Ιωσήφ
Abstract The efforts to functionally inhibit proteases in apicomplexans by using NcPI-S serpin defined a novel strategy to generate genetically attenuated parasites, which is currently extended towards two directions in our laboratory. The first direction is to use molecularly engineered serpins in order to block proteolysis in invasion related organelles and the other is to test the inhibitory potential of additional naturally derived serpins against proteolytic machineries of apicomplexan parasites by using Plasmodium berghei ookinetes as a test tube system. We decided to target molecular engineered MPIs in the micronemes of Toxoplasma tachyzoites aiming to inhibit critical proteolytic steps that take place in those organelles and we also attempted to develop a one step cloning transfection vector, which would facilitate one step sub-cloning of natural or molecular engineered MPIs and the subsequent generation of transgenic P. berghei parasites expressing those MPIs at the ookinete stage. In this case a high throughput screen would develop allowing us to identify serpins interfering with ookinete midgut invasion and ookinete to oocyst transition.
Language English
Subject Ακροσυμπλεγματικά
Issue date 2010-11-19
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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