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Identifier 000454831
Title The TuReLi TTX assay plate : Development of a Tunable and Reversible Linear Thermal Gradient Plate based on open-source modules for C. elegans thermotaxis assays
Alternative Title The TuReLi TTX assay plate
Author Σπινθάκη, Σοφία
Thesis advisor Μπαζοπούλου, Δάφνη
Reviewer Πανταζής, Αλέξανδρος
Abstract Thermal gradient platforms are used in a range of response testing applications from materials and components characterization i.e., glass transmission T oC, melting point, MFFT, to biology research. Thermotaxis (TTX) is the organism’s locomotion strategy in response to environmental temperature changes or stimuli. This behavior ensures that organisms will locate themselves at optimal conditions and is the subject of nervous system studies. In the popular model organism Caenorhabditis elegans, TTX assays are employed for learning and memory studies i.e., how environmental signals shape behavioral output, with regards to previous experiences. During those assays, C. elegans navigate towards preferable isothermals when traveling up and down a thermal gradient. TTX assays require technical components that provide sensitivity, stability, high-resolution and/or high throughput capabilities. Commercially available equipment exists and requires minimum user expertise. However, this type of equipment drastically increases the cost while limiting potential output, since it is usually not compatible with other lab equipment. For that reason, research labs are turning into customized platforms that are tailored to their in house setup but have no provision for implementation from other users. Here, we developed a stand-alone, tunable TTX device: the TuReLi (Tunable, Reversible and Linear) TTX assay plate, from 3D printed parts and readily available modules, which allows high resolution multi-position temperature control and recording. Our system is open source-based, affordable to fabricate and requires no industrial control units. Due to the flexible and user-friendly operation, the TuReLi TTX is an ideal, universal solution for thermotaxis-based assays.
Language English
Issue date 2023-04-05
Collection   School/Department--School of Medicine--Department of Medicine--Post-graduate theses
  Type of Work--Post-graduate theses
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