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Identifier 000441521
Title Development of obligate fermentative Escherichia coli srains
Alternative Title Ανάπτυξη υποχρεωτικά ζυμωτικών στελεχών Escherichia coli
Author Ανδρεαδάκη, Θεοφανία Παγώνα Ι.
Thesis advisor Γανωτάκης, Δημήτριος
Reviewer Τσιώτης, Γεώργιος
Παυλίδης, Ιωάννης
Abstract The aim of this work was the development and study of obligate fermentative Escherichia coli strains. An obligate fermentative strain derived from SIJ488 was studied both in the presence of various single carbon sources such as glucose, glycerol, acetate, pyruvate, and in a combination of glucose with pyruvate. This new strain was furtherly evolved, providing interesting sequencing information, and thus contributing to the deeper understanding of its fermentative metabolism with the support of techniques such Reverse Transcriptase-quantitative PCR. Νew strains were generated either to mimic the evolution or to provide deeper understanding of the metabolism of the mutated strains. λ- Red Recombination was used for the gene deletions. The new strains were studied in the presence of various carbon sources such as glucose, glycerol, acetate, or pyruvate. Ion exchange chromatography was used for the quantification of the fermentation products and nuclear magnetic resonance analysis was used for additional product confirmation. Morphological studies by Scanning Electron Microscopy and negative staining of the strains also carried out. Thus, information was acquired on how their engineered metabolism affects the morphology of the cell by causing shape elongation and loss of flagella. Finally, another two obligate fermentative strains were developed with mostly the gene deletion method P1- Phage transduction.
Language English
Subject Aerobic conditions
Electron transport chain
Escherichia coli SIJ488
Ion Chromatography
Αερόβιες συνθήκες
Αλυσίδα μεταφοράς ηλεκτρονίων
Γαλακτικό οξύ
Ιοντική χρωματογραφία
Issue date 2021-07-30
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
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