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Identifier 000426448
Title Molecular, genetic and immunolocalization approaches for analyzing insecticide resistance in major agricultural pests
Alternative Title Μοριακές και γενετικές προσεγγίσεις και μελέτη ανοσοεντοπισμού για την ανάλυση της ανθεκτικότητας σε κύριους εχθρούς καλλιεργειών
Author Σκουφά, Ευαγγελία Δ.
Thesis advisor Χαλεπάκης, Γεώργιος
Abstract Tetranychus urticae is one of the most economically important and damaging pest in agriculture worldwide. Its high fecundity, arrhenotokous reproduction and short life cycle in combination with intensive chemical spraying lead to limited control of this pest. In the present survey, the resistant levels of a Greek Tetranychus urticae population, derived from Trizinia, against a range of chemicals with different mode of action were investigated. Trizinia population exhibited high resistance levels against pesticides of different chemical classes. Moreover, the molecular mechanisms underlying abamectin resistance were also examined. By using genetic crosses, toxicity bioassays genotyping we investigate involvement of a novel mutation (I310T) in the GluCl3 in abamectin resistance. In parallel, lack of knowledge for tissues implicated in detoxification physiology led us to investigate the localization pattern of detoxification enzymes within the organism. Delta GSTs and CYP392A16 are detoxification enzymes directly associated with cyflumetofen and abamectin resistance in T. urticae, respectively. Immunofluorescence experiments indicated that both of these enzymes were located mainly in midgut. In addition, delta GSTs were also expressed underneath the cuticle. In the last chapter of this thesis, the frequency of A2001V mutation in ACCase of Bemisia tabaci, strongly associated with ketoenol resistance, was monitored in whitefly populations collected from different Mediterranean countries. Additionally, a simple PCR-RFLP molecular diagnostic was developed and may be applied as a valuable tool for the early and accurate detection of the mutation in the field.
Language English
Subject Ανοσοεντοπισμός
Issue date 2019-11-29
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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