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Identifier

000373187

Title

Γενετική και μοριακή ανάλυση ενός νέου ρόλου της πρωτεϊνης Chk1 : η πρωτεϊνη Chk1 απαιτείται για μείωση των μεροτελικών προσδέσεων και των χρωμοσωμάτων που καθυστερούν κατά τη διάρκεια της κυτταρικής διαίρεσης / Ελένη Πετσαλάκη; επιβλέπων καθηγητής και επιστημονικός υπεύθυνος καθ. Γεώργιος Ζάχος.

Alternative Title

Genetic and molecular analysis of a novel Chk1 function

Author

Πετσαλάκη, Ελένη

Thesis advisor

Ζάχος, Γεώργιος

Abstract

Correction of improper kinetochore-microtubule attachments is critical for accurate chromosome segregation. Merotelic attachments in which a single kinetochore binds to microtubules emanating from both spindle poles occur frequently during the early stages of mitosis, and if not corrected prior to anaphase, can lead to lagging chromosomes and aneuploidy. Merotelic attachments are not detected by the mitotic spindle checkpoint and the mechanism of their correction is not fully understood. In the present study, we show that conserved protein kinase Chk1, a well-established component of the DNA damage response, is required to reduce merotelic kinetochore attachments and anaphase lagging chromosomes during mitotic cell division in vertebrate cells. We propose that Chk1 regulates correction of merotelic attachments during a prolonged metaphase. We also propose that Chk1 is required for optimal Aurora-B phosphorylation and activity during repair of merotelic attachments in metaphase cells. We will further investigate this novel role of Chk1 in regulating fidelity of kinetochore-microtubule interactions.

Language

Greek

Subject

Merotelic attachments

Mitosis

Μίτωση

Issue date

2010-11-19

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses

Type of Work--Post-graduate theses

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