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Identifier

000362042

Title

Καταγραφή αλληλομόρφων ανθεκτικότητας Ops και Pyr σε πληθυσμούς του B. tabaci της Κρήτης και της Κύπρου

Alternative Title

Monitoring of resistance B. tabaci alleles to OPs and Pyr from Crete and Cyprus

Author

Περράκης, Ανδρέας Κων.

Thesis advisor

Βόντας, Ιωάννης
Τσαγκαράκου, Αναστασία

Abstract

Simple PCR-agarose gel visualization based assays for reliably monitoring the Pyrethroid and Organophosphate resistance-associated mutation have been developed in the B. tabaci (Gennadius). Samples were collected from highly productive areas from both East and West parts of Crete and from eight fields from Cyprus. In the present study we apply PCR-RFLP assays for detecting the L925I and F331W mutations in the B. tabaci para-type voltage gated sodium channel gene and the acetylcholinesterase enzyme ace1 respectively. A highly specific PASA was used for detecting the T929V mutation found in the B. tabaci of B biotype. Those tests were used to monitor the frequency of the resistance mutations of the Β biotype from Cyprus and the Q biotype from Crete. The F331W mutation was fixed in all fields individual examined. The mutated Kdr alleles I925 (r1) and V292 (r2) conferring resistance to Pyr were uniformly distributed across the geographical sampling area of East and West Crete, but not across the East part, between Tympaki and Ierapetra. In Cyprus the mutated Kdr alleles were not uniformly distributed across the geographical sampling area.

Language

Greek

Subject

Bemisia tabaci

Biotype B, Q

Ανθεκτικότητα

Κρήτη

Κύπρος

βιότυπος B,Q

Issue date

2010-11-19

Collection