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Identifier 000461081
Title Deciphering molecular regulators of neuroinflammation in Alzheimers disease using iPSC-derived microglia
Alternative Title Αποκωδικοποιώντας τους μοριακούς ρυθμιστές της νευροφλεγμονής στη νόσο Αλτσχάιμερ με τη χρήση επαγόμενων πολυδύναμων βλαστικών κυττάρων μικρογλοίας
Author Λυγερούδη, Ανίτα
Thesis advisor Σάλτα, Ευγενία
Reviewer Penning, Amber
Snoeck, Sarah
Abstract Alzheimer’s disease (AD) is a fatal neurodegenerative disease with no available treatment. Since the generation of induced pluripotent stem cells (iPSCs), several cell-based models of different diseases have been developed. In an attempt to better understand the disease aetiology and identify effective therapeutics for AD, human iPSC lines have been utilised to study both the familial (fAD) and the sporadic (sAD) form of AD. However, there has not yet been a systematic and detailed characterization of microglia derived from human sAD iPSC lines at baseline. By monitoring gene expression profiles using quantitative PCR and immunofluorescent labelling, we focus on the characterization of two iPSC lines derived from healthy individuals and one iPSC line derived from a patient with sAD. We found that iPSCderived microglia highly express microglial markers, thereby validating their cellular identity in our experimental setting. In addition, we showed an upregulation of DAM and AD risk genes in the sAD iPSC line, and we observed distinct morphological characteristics in the sAD iPSC line compared to controls, suggestive of a more activated phenotype, recapitulating microglial behavior in AD brain. Interestingly, despite these observations no downregulation of miR-132 expression levels in the sAD iPSC line was observed. This study will provide fundamental knowledge for future reference, to better evaluate prospective results under different treatment conditions.
Language English
Subject Baseline characterisation
Induced pluripotent stem-cells
Sporadic Alzheimers disease
Σποραδική νόσος Αλτσαχάιμερ
Issue date 2023-12-08
Collection   School/Department--School of Medicine--Department of Medicine--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/3/6/7/metadata-dlib-1702036009-538872-21299.tkl Bookmark and Share
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