Your browser does not support JavaScript!

Home    Collections    Type of Work    Post-graduate theses  

Post-graduate theses

Search command : Author="Κούτρα"  And Author="Αικατερίνη"

Current Record: 20 of 6472

Back to Results Previous page
Next page
Add to Basket
[Add to Basket]
Identifier 000460577
Title Morphological and functional characterization of cortical interneurons in Transgenic mice
Alternative Title Μορφολογικός και λειτουργικός χαρακτηρισμός ενδονευρώνων του εγκεφαλικού φλοιού σε διαγωνιδιακούς μύες
Author Σηφάκης, Ιάσων
Thesis advisor Καραγωγέως, Δόμνα
Reviewer Βιδάκη, Μαρία
Σιδηροπούλου, Κική
Abstract Cortical interneurons (CINs), despite constituting a minority of cortical neurons, play critical roles in cortical function and have been implicated in neurological disorders. CINs exhibit remarkable diversity in morphology, molecular markers, and function, reflecting the complexity of their development. This thesis explores the role of the small GTPase Rac1 in the maturation of CINs, with a specific focus on somatostatin-expressing (SST+) CINs and the role of TAG-1/CNTC-2 in the myelination of SST+ CINs and their functions. Using a genetically engineered mouse model with Rac1 selectively ablated in medial ganglionic eminence (MGE)-derived CINs, we investigated the morphological changes during early postnatal development. Notably, we observed a significant reduction in the soma perimeter of SST+ CINs, suggesting a role for Rac1 in cytoskeletal dynamics. Additionally, we assessed synapse formation in these CINs. Immunocytochemistry revealed a decrease in both inhibitory and excitatory synapse density on the soma and primary dendrites of SST+ CINs lacking Rac1. Given the extensive connections between pyramidal cells and SST+ interneurons, we propose further investigations into potential alterations in synapses on pyramidal cells in Rac1-deficient mice. While Rac1's involvement in CIN development is well-documented, its precise role in modulating the morphology and synaptogenesis of SST+ CINs during early postnatal stages adds a new layer of complexity. Potential downstream molecules and differential effects in distinct neuronal populations warrant further exploration. Regarding the TAG-1/CNTC-2 mouse line, electrophysiological recordings show altered potentiation of the CA1 circuit, where our findings reveal a stark difference between TAG-1/CNTN-2 knockout and wild-type animals, suggesting the involvement of intricate inhibitory-excitatory interactions and potential variations in NMDA receptor function. This thesis offers a comprehensive journey into the world of CINs, unraveling their complex development and functional significance. The insights gained not only expand our understanding of neural circuitry but also open avenues for further exploration into the molecular mechanisms that underlie cortical function.
Language English
Subject Morphology
Synapses
Ενδονευρώνες
Μορφολογία
Συνάψεις
Issue date 2023-12-08
Collection   School/Department--School of Medicine--Department of Medicine--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/e/9/2/metadata-dlib-1700823748-874419-22944.tkl Bookmark and Share
Views 1022

Digital Documents
No preview available

Download document
View document
Views : 1