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Identifier

000361624

Title

Homologous association of the mouse TNFa locus

Alternative Title

Ομόλογη αλληλεπίδραση του γενετικού τόπου του TNFa στο ποντίκι

Author

Στρατήγη, Καλλιόπη Χ.

Thesis advisor

Σπηλιανάκης, Χ.
Καρδάσης, Δημήτριος

Abstract

Lipopolysaccharide (LPS) stimulation of murine macrophages triggers the activation of signalling pathways that lead to the nuclear translocation of Nf-θB and the subsequent expression of TNFα., among other pro-inflammatory cytokines. In the present project we have studied the events that take place in the cell nucleus before and during the expression of TNFα and, specifically, we aimed in identifying the protein factors that regulate the inter-chromosomal interactions between the two murine TNF alleles. We found that the homologous association of the two TNFα alleles takes place 30min upon the stimulation of macrophages with LPS. The highest level of TNF expression followed this homologous interaction, at 1h upon LPS stimulation and accordingly the simultaneous transition from mono- to by-allelic expression was detected at the same time point. In correspondence to the model of transvection and the GAGA factor that mediates homologous pairing in Drosophila, we used a series of molecular and genetic approaches to identify the protein complexes that regulate these long-range interactions in mammals.

Physical description

39 σ. : πιν. ; 30 εκ.

Language

English

Subject

Cell Physiology

Homologous

TNFa

Ομόλογη

Issue date

2009-12-14

Collection