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Identifier

000397275

Title

Analysis of a novel transgenic mouse line with defective fiber tracts

Author

Στρατήγη, Αικατερίνη Ε.

Thesis advisor

Καραγωγέως, Δόμνα

Abstract

The function of the cerebral cortex relies on several stereotypical long-distance projections, which originate from excitatory projection neurons that represent the largest portion of all cortical neurons. Axonal tracts, originating from projection neurons connect areas essential for brain function and they are also important for the final structure of the central nervous system. Cortical efferents (corticothalamic axons or CTAs) are expressing high levels of Transient Axonal Glycoprotein-1 or Contactin-2 (TAG-1/Cntn2), a neuronal recognition molecule of the immunoglobulin superfamily which is involved in neurogenesis, neurite outgrowth and fasciculation. Among other neuronal subpopulations, it is expressed early by pioneer neurons in the preplate and later on in the marginal zone and subplate of the developing cortex. To study the formation of several axonal tracts in the mouse central nervous system, we generated the transgenic mouse line Tag1loxP-GFP-loxP-DTA. These mice express GFP under the Tag-1 promoter, also encompassing the coding sequence of Diptheria Toxin subunit A (DTA) under quiescence. Upon crossing with the neocortex-specific Emx1::Cre line, GFP expression is eliminated and the toxin is expressed in TAG-1+ neurons cells resulting in their death. Cortical lamination deficits and aberrant axonal connectivity in the brain is a main cause of neurodevelopmental disorders in human. Emx1::Cre;Tag-1::DTA mice have reduced CTAs and their analysis in embryonic and postnatal stages will give useful information for the role of these axons in the brain development.

Language

English

Subject

Circuits development

Neural circuits

Issue date

2015-11-20

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses

Type of Work--Post-graduate theses

Permanent Link

https://elocus.lib.uoc.gr//dlib/f/d/2/metadata-dlib-1448274305-420095-32263.tkl