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Doctoral theses
Current Record: 2152 of 2435
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| Identifier | 000333624 | ||||||
| Title | Μηχανισμοί πλαστικότητας στον ενήλικο εγκέφαλο του Zebrafish (Danio rerio) | ||||||
| Alternative Title | Plastisity mechanisms in adult Zebrafish (Danio rerio) brain: sexual differentiations | ||||||
| Author | Αμπατζής, Κωνσταντίνος Σ | ||||||
| Thesis advisor | Κεντούρη, Μαρουδιώ | ||||||
| Abstract |
Binding sites were observed to be heterogeneously distributed throughout the brain. In both sexes dense α 2- ARs were observed in the locus coeruleus (LC), magnocellular octaval nucleus (MaON) and dorsal telencephalic (Dc, Dp) nuclei. However, β-ARs were found in dense binding in LC, in all cerebellar subdivisions, in dorsal zone of periventricular hypothalamus (Hd), and in ventral telencephalic nuclei. In addition we performed immunohistochemical studies to reveal the regional distribution of α 2A-ARs, β 2-ARs and adrenaline in the adult zebrafish brain. Detailed mapping showed labeling of α2A-ARs, in neuropil, neuronal somata and fibers, glial processes and blood vessels. High density of α2A-AR, β2-ARs and adrenaline immunoreactivity was found in the ventral telencephalic area, preoptic, pretectal, hypothalamic areas, torus semicircularis, oculomotor nucleus (NIII), locus coreruleus (LC), medial raphe, medial octavolateralis nucleus (MON), magnocellular octaval nucleus (MaON), reticular formation (SRF, IMRF, IRF), cerebellar Purkinje cell layer and especially α2A-ARs found in rhombencephalic nerves roots (DV, V, VII, VIII, X). Moderate levels of α2A-ARs, β2-ARs and adrenaline were observed in the dorsal telencephalic area, in the periventricular gray zone of optic tectum, in the dorsomedial part of optic tectum layers, in the molecular and granular layer of all cerebellum subdivisions. Glial processes were found to express α2A-ARs in rhombencephalon, intermingled with neuronal fibers. Medium size neurons were labelled in telencephalic, diencephalic and mesencephlic areas, while densely labelled large neurons were found in rhombencephalon, in the locus coeruleus, the reticular formation, the oculomotor, the medil octavolateralis and magnocellular octaval nuclei and Purkinje cell somata. From double labelled experiments also both neuronal and glial ocalization of β2ARs and adrenaline were revealed. Dimorphisms in the density of α2- ARs binding was identified in dorsal telencephalic Dl nucleus, preoptic PPa and PPp nuclei, hypothalamic nucleus (Hd), central gray (GC), and in granular layer of corpus and caudal lobe of cerebellum (CCe gr, LCa gr). In all these areas the females showed higher [3H]RX821002 binding than males. However, only four areas showed sex dimorphisms in the density of β-ARs, the Hd, GC, CCe gr and LCa gr. In Hd and GC females showed higher [3H] CGP12177 binding levels than males, while males demonstrated higher levels than females in CCe gr and LCa gr. The localization of [3H]RX821002 and [3H] CGP12177 binding sites and the sex dimorphisms in adrenoceptor densities in particular brain regions, indicates that the adrenergic/noradrenergic system could be associated with the control of essential functions as sex determination, reproductive behaviour, and other sex-specific behaviours. |
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| Language | Greek | ||||||
| Subject | brain zebrafish | ||||||
| plasticity mechanisms | |||||||
| εγκέφαλος zebrafish | |||||||
| μηχανισμοί πλαστικότητας | |||||||
| Issue date | 2008-09-25 | ||||||
| Collection | School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses | ||||||
| Type of Work--Doctoral theses | |||||||
| Permanent Link |
https://elocus.lib.uoc.gr//dlib/c/f/9/metadata-dlib-aca860212d02ffb7ab46409ad7033416_1233124449.tkl
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| Views | 396 | ||||||
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