Doctoral theses
Current Record: 2119 of 2443
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| Identifier |
uch.med.phd//2001giannikaki |
| Title |
Ανοσοϊστοχημική μελέτη των ογκοπρωτεϊνών BCL-2 και MDM2 της ογκοκατασταλτικής πρωτεΐνης του κυτταρικού κύκλου P21 σε καρκινώματα μαστού. Συσχετισμός τους με την ιστολογική διαφοροπίηση, την ύπαρξη λεμφοδενικών μεταστάσεων και του δείκτη κυτταρικού πολλαπλασιασμού MIB-1 |
| Creator |
Giannikaki-Vagiou, Elpida
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| Abstract |
The aim of the present study was to investigate the pattern of expression of the two wild-type (wt) p53 induced proteins mdm-2 and p21/ waf1 and bcl-2 anti-apoptotic protein in breast carcinomas in relation to the expression of p53 protein, the c-erbB-2 oncoprotein, the oestrogen receptor status, the proliferation nuclear antigen MIB1/ Ki-67, the histological grade and the lymph node status in order to obtain combined information of these proteins in the genetic pathway of breast carcinoma and to estimate the possible clinical use of these proteins in evaluating the biological behaviour of different immunotypes characterizing these tumors. Formalin-fixed paraffin embedded tissue from 144 breast carcinomas was stained by immunohistochemistry for p53, mdm2, p21, c-erbB2, bcl-2 proteins, estrogen receptors (ER) and MIB1/ Ki-67. We found p53, p21, mdm2 and bcl-2 protein expression in 59/144, 53/144, 35/144 and 61/144 carcinomas, respectively. Parallel expression of p53/ mdm2 protein was found in 20 cases. 11 were also p21 positive. Discordant p53+/mdm2- protein expression was found in 39 cases. 18 of these were p21 positive and 21 were p21 negative. 54 cases were p53-/mdm2-/p21- and 8 were p53-/ mdm2-/ p21+. The expression of p21 in breast carcinomas may be due to normal or increased activation of wt-p53, while over-expression of p53 is related with co-expression of mdm2 protein. The p21 expression is related with carcinomas with a more aggressive behavior, increased mitotic activity and ER+. The mdm-2 expression is related with high grade carcinomas and ER+. The immunotypes p53+/mdm2+ and p53+ /p21- are related with lymph node metastasis and high grade neoplasms. Bcl-2 expression had a tendency for a discordant relation with p53, p21 and mdm-2 proteins expression. But there was a statisticaly significant relationship with the c-erb-B-2 expression.
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| Issue date |
2001-05-01 |
| Date available |
2001-08-02 |
| Collection
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School/Department--School of Medicine--Department of Medicine--Doctoral theses
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Type of Work--Doctoral theses
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| Permanent Link |
https://elocus.lib.uoc.gr//dlib/c/8/a/metadata-dlib-2001giannikaki.tkl
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| Views |
320 |
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