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Identifier uch.biology.phd//1996DIS0004
Title Το ανθρώπινο νευρωνικό μόριο συνάφειας ΤΑΧ-1: Κλωνοποίηση και ανάλυση της δομής και της λειτουργίας του
Alternative Title Cloning of the human neuronal cell adhesion molecule TAX-1: structural and functional studies
Author Τσιότρα, Παναγιούλα Χ
Thesis advisor Παπαματθαιάκης, Ιωσήφ
Abstract The present study describes the cloning and functional analysis of the human neural cell adhesion molecule TAX-1, as the chromosomal organization and regulation of the TAX-1 gene. Screening of a human cerebellum CDNA library, led to the isolation of the TAX-1 CDNA molecule, that showed 91% and 75% similarity at the amino acid level with its homologues TAG-1/F3 subgroup of the IG superfamily of neural cell adhesion molecules, that is six(6) immunoglobulin like domains of the C2 type(IGC2) which are followed by four(4) fibronectin type III repeats(FNIII) and a C-terminal hydrophobic region, characteristic to proteins that are linked to the cell membrane via a GPI linkage. The second IGC2-type domain presents the highest similarity among the three homologous proteins, whereas only the two mammalian preteins(TAG-1 and TAX-1)share in the second FNIII-type repeat the RGD tripeptide, a motif known to mediate recognition of fibronection by integrins. Stable expression of the TAX-1 molecule in the non-adherent cell line of D.Melanogaster Schneider-2(S2), showed a dramatic increase of cell adhesion in the transfected S2 cells. In analogous experiments using deletion constructs expressing either the IGC2-type domains or the FNIII-type repeats of the TAX-1 protein, we have shown that the FNIII repeats alone, are both sufficient and necessary for mediating the homophilic adhesive properties of TAX-1 protein(Abstract truncated).
Language Greek
Subject Ανοσοσφαιρινικές περιοχές; ΓΛΥΚΟΖΥΛ-ΦΩΣΦΑΤΙΔΥΛ-ΙΝΟΣΙΤΟΛΙΚΟΣ(GPI) ΔΕΣΜΟΣ; ΙG ΥΠΕΡΟΙΚΟΓΕΝΕΙΑ ΤΩΝ ΚΥΤΤΑΡΙΚΩΝ ΜΟΡΙΩΝ ΣΥΝΑΦΕΙΑΣ (CAMS); ΝΕΥΡΩΝΙΚΟ ΜΟΡΙΟ ΣΥΝΑΦΕΙΑΣ; ΟΜΟΦΙΛΙΚΗ ΣΥΝΑΦΕΙΑ /ΑΛΛΗΛΕΠΙΔΡΑΣΗ; ΦΙΜΠΡΟΝΕΚΤΙΝΙΚΕΣ ΠΕΡΙΟΧΕΣ
Issue date 1996-03-07
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Doctoral theses
  Type of Work--Doctoral theses
Permanent Link https://elocus.lib.uoc.gr//dlib/f/0/a/metadata-dlib-1996DIS0004.tkl Bookmark and Share
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