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Identifier 000424383
Title A point-of-care, microfluidic-based microscanner for the identification and counting of single cells
Alternative Title Σύστημα μικρού όγκου ,οπτικής σάρωσης μικρορευστονικού καναλιού για την ταυτοποίηση και ανίχνευση μεμονωμένων κυττάρων στο σημείο περίθαλψης
Author Κορομπίλη, Γεωργία
Thesis advisor Χρόνης, Νικόλαος
Reviewer Χατζηνικολαϊδου, Μαρία
Αλεξόπουλος, Λεωνίδας
Τζωρτζάκης, Στέλιος
Κιοσέογλου, Γεώργιος
Μισιάκος, Κωνσταντίνος
Γκιζελή, Ηλέκτρα
Abstract Blood cell counting is related to the diagnosis and therapy of various diseases – infections, blood disorders, cancer treatment. However, the time consuming procedures and the increased cost of standard medical equipment act as serious obstacles in the early screening of a disease. To meet this challenge, there is an emerging trend towards a patient-centered healthcare that provides medical examination and diagnosis at the point of care. Profiting from recent advances in Micro-ΕlectroΜechanical systems, micro-optics and micro-fluidics, the present thesis attempts to present a platform technology for blood cell identification and counting at the point of care. The developed microscanner consists of 3 novel components: (1) a microlens array enabling extended sample imaging with single cell resolution in trans-illumination imaging scheme (2) a novel design, integrating a micro-LED array and a mini-lens array on the same silicon chip for epi-illumination sample imaging and (3) a microfluidic chip for specific cell capturing from undiluted, whole blood samples. The characterization process of the individual subsystems demonstrates: (a) high optical resolution (1μm) under conditions of maximum magnification in trans-illumination scheme; (b) the ability to screen a large sample surface (2.5 cm2); (c) the achievement of 8.8μm resolution in reflection based scheme; (d) the reduction of the structure’s optical length below 2.5 mm and (e) the achievement of a clinically negligible error in white blood cell capturing in the microfluidic device, below 3.8%, compared with flow cytometry. The developed system constitutes a platform technology; it is applicable to various diseases. The conducted research proves the feasibility of single cell identification and counting in full compliance with the requirements of World Health Organization for point-of-care devices: they need to be affordable, easy-to-use, battery operated and deliverable unconditionally to all.
Language English
Subject Cell fractionation
Micro-led on silicon
Microfluidic design
Microsystem design
Mini-lens array
Miniaturized microscopes
Reflection -based imaging
Απεικόνιση ανακλώμενου φωτός
Απομόμωση κυτταρικών πληθυσμών
Μικρορευστονικές διατάξεις
Μικροσυστήματα μικροσκοπίας
Μικρό-led σε πυρίτιο
Συστοιχία μικρό-φακών
Σχεδιασμός μικροσυστημάτων
Issue date 2019-11-29
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Materials Science and Technology--Doctoral theses
  Type of Work--Doctoral theses
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