E-Locus - Ιδρυματικό Καταθετήριο Πανεπιστημίου Κρήτης

Αρχική Συλλογές Τύπος Εργασίας Διδακτορικές διατριβές

Διδακτορικές διατριβές

Τρέχουσα Εγγραφή: 7 από 2007

[Προσθήκη στο καλάθι]

Κωδικός Πόρου

000426362

Τίτλος

Regulation of gene expression in cis and in trans

Άλλος τίτλος

Ρύθμιση της γονιδιακής έκφρασης σε cis και trans

Συγγραφέας

Piwko, Pawel S.

Σύμβουλος διατριβής

Δελιδάκης, Χρήστος

Μέλος κριτικής επιτροπής

Σπηλιανάκης, Χαράλαμπος
Καλαντίδης, Κρίτων
Αλεξανδράκη, Δέσποινα
Παπαματθαιάκης, Ιωσήφ
Τζαμαρίας, Δημήτριος
Γαρίνης, Γεώργιος

Περίληψη

bHLH-Orange proteins, like the mouse Hes and the Drosophila Enhancer of split [E(spl)] proteins are transcription factors that, among a multitude of functions, are implicated in maintaining the undifferentiated state in many stem-cell based systems. For instance, they antagonize bHLH proneural activators and suppress neural fate. Their levels have to be exquisitely regulated to achieve a precise balance between proliferation and differentiation. For this reason they are subject to multi-tiered regulation. During my PhD research I have tried to understand how the expression of two neighboring E(spl) genes, E(spl)m7 and E(spl)m8, is regulated and how this regulation impacts biological processes controlled by their protein products. I have provided description of their enhancer, promoter and 3' untranslated elements and scrutinized the role of these elements in specifying spatio-temporal abundance of the E(spl)m7 and E(spl)m8 proteins. I demonstrate that compromising transcriptional negative autoregulation and translational repression can 2 have dramatic consequences on the expression levels and the activity of these two genes. Thus, both regulatory modes are essential in determining level-dependent functions of E(spl)m7 and E(spl)m8.

Φυσική περιγραφή

iii, 118 φύλλα : πίν., σχήμ., εικ.(μερ. εγχρ.) ; 30 εκ.

Γλώσσα

Αγγλικά

Θέμα

Drosophila

E(SPL)

Homologous pairing

Transgenesis

Transvection

Ε(ΣΠΛ)

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