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Identifier

000379234

Title

Identification and characterization of genes involved in resistance to neonicotinoid insecticides in Drosophila melanogaster

Alternative Title

Ταυτοποίηση και χαρακτηρισμός γονιδίων που ενέχονται στην ανθεκτικότητα σε νεονικοτινοειδή εντομοκτόνα στη Drosophila melanogaster

Author

Kalajdzic, Predrag Milan

Thesis advisor

Σαββάκης, Χαράλαμπος

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Καραγωγέως, Δόμα
Δελιδάκης, Χρήστος
Μαυροθαλασσίτης, Γεώργιος
Βόντας. Γιάννης
Ηλιόπουλος, Γιάννης
Μαθιόπουλος, Κώστας

Abstract

Insecticide resistance is a serious, long term problem that impacts agricultural production and health of animals and humans. Resistance to all major insecticide classes, including neonicotinoids, arose in numerous and diverse insect field populations. Imidacloprid, the most prominent neonicotinoid, has been widely used during the last decade in controlling different insect pests. Drosophila melanogaster, although not a pest species, is a widely used model organism and a promising model system for insecticide resistant research. In our study we have analyzed a Drosophila laboratory mutant which is resistant to Imidacloprid and cross-resistant to DDT. The mutant has been retrieved in a genome-wide Minos-based insertional mutagenesis screen. The resistant line was characterized using genetic, toxicological, molecular and transriptomic analysis. Genetic analysis mapped resistance to the right arm of the second chromosome. Toxicologal analysis showed higher activity of P450 enzymes, while molecular analysis revealed higher expression of three unlinked P450 genes in the resistant line compared to the susceptible line. Deep sequencing transcriptomic analysis showed changes in several groups of genes involved in metabolic processes. Taken together, these results strongly suggest that the mutation results in upregulation of several unlinked genes involved in metabolic detoxification of the insecticides. The exact molecular mechanism remains to be elucidated.

Language

Greek, English

Subject

Deep sequencing

Drosophila melanogaster

Genetic mapping

Insecticide Resistance

Insecticides

Insertional mutagenesis

Molecular Biology

Αλληλουχία

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