Doctoral theses
Current Record: 2102 of 2446
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| Identifier |
335437 |
| Title |
Μελέτη της αγγειογένεσης στην οξεία λεμφοβλαστική λευχαιμία της παιδικής ηλικίας |
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Author
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Καλμαντή, Λήδα
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| Abstract |
Angiogenesis has been shown to play a key role in the growth of solid tumors. Recently, a similar role has been proposed for hematologic malignancies. Relative studies have been performed predominantly in adults, based on the principle of the Hot Spot counting. Considering the implication of angiogenesis in leukemias of childhood, we designed this study. The specific aim of the study was to develop a precise, of clinical importance, quantitative method for estimating bone marrow microvasculature both in normal and neoplastic bone marrow. We used acute lymphoblastic leukemia as a model, and we tested the quantitative results at the onset of disease. Formalin-fixed and paraffin-embedded bone marrow biopsies from 26 newly diagnosed children with acute lymphoblastic leukemia (mean age 6.44 ) were immunostained for factor VIIIRAg and compared with those from 27 children ( mean age 8.5 ) with no evidence of bone marrow disease, used as controls. Immunohistochemical detection of microvasculature was reached using a standard alkaline phosphatase method with fast red as chromogen. Microvessel density (MVD) was evaluated at the area containing the highest number of microvessels (hot spots) and additionally by the VC-MDI on the whole microscopic section of the bone marrow biopsy. The volume corrected microvessel index (VC-MDI) equates the number of microvessels per fraction of both neoplastic and normal bone marrow cells (NBMC/NBMC+fat cells ). Overall both MVD and VC-MVDI were increased at diagnosis of Acute lymphoblastic leukemia in comparison with the control group ( MVD 6.2± 3.79 , 1.3±0.16 p < 0.05). However the VC- MVDI increment (VC- MVD1 0.94 ± 0.169 , 0.7±0.18 p >0.05) was not statistically significant. Therefore we propose that angiogenesis in childhood ALL should be evaluated by VC- MVDI since it is more representative and subjective.
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| Language |
Greek |
| Issue date |
2005-12-01 |
| Date available |
2006-09-26 |
| Collection
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School/Department--School of Medicine--Department of Medicine--Doctoral theses
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Type of Work--Doctoral theses
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| Permanent Link |
https://elocus.lib.uoc.gr//dlib/c/5/e/metadata-dlib-2005kalmanti.tkl
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| Views |
263 |
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