Doctoral theses

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Identifier	322584
Title	Το μεταθετό στοιχείο Minos:Ανάπτυξη συστημάτων για λειτουργική γενωμική ανάλυση στον άνθρωπο και στον ποντικό
Creator	Zagoraiou, Laskaro
Abstract	The Drosophila hydei transposable element Minos belongs to the mariner-Tc1 superfamily of transposons, members of which are found in diverse eukaryotic organisms. Many members of the superfamily can transpose in organisms evolutionarily distant to the host they were originally isolated from. The only requirements for transposition appear to be the presence of active transposase and intact inverted repeats. In this thesis, the ability of the Minos element to transpose in somatic cells and in the germ line of the mouse (Mus musculus) from one chromosomal position to a new one is demonstrated. This establishes a valuable tool towards functional genomic analysis in the mouse. Furthermore, mouse lines carrying an inducible transposase production system were created. It has previously been demonstrated that the transposable element Minos is a valuable tool for saturating insertional mutagenesis of the genome of HeLa cells. For insertional saturation of cell lines that do not take up DNA efficiently, a large number of transfections would be required. To circumvent this problem, the Minos system was combined with a virus which enters mammalian cells but does not replicate in them (AcNPV, baculovirus). Chimeric Minos-AcNPV vectors were established. Use of these vectors increased the number of clones carrying transposon insertions in various mammalian cell lines, opening the way for functional genomic experiments in many cell types.
Language	Greek
Issue date	2004-12-01
Date available	2006-08-04
Collection	School/Department--School of Medicine--Department of Medicine--Doctoral theses
	Type of Work--Doctoral theses
Permanent Link	https://elocus.lib.uoc.gr//dlib/c/a/d/metadata-dlib-2004zagoraiou.tkl
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