Doctoral theses
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| Title |
Ανάπτυξη και εφαρμογή γενετικών εργαλείων με στόχο τη μελλοντική βελτίωση της παραγωγής σε δύο νέα είδη της Μεσογειακής ιχθυοκαλλιέργειας, τον κρανιό (Argyrosomus regius) και το μαγιάτικο (Seriola dumerili) |
| Alternative Title |
Development and application of genetic tools aiming at the future production improvement in two new species for the mediterranean aquacultiure, the meagre (Argyrosomus regius) and the greater amberjack (seriola dumerili) |
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Author
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Νούσιας, Ορέστης
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Thesis advisor
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Παυλίδης, Μιχαήλ
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Reviewer
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Τσιγγενόπουλος, Κωνσταντίνος
Δελιδάκης, Χρήστος
Σπηλιανάκης, Χαράλαμπος
Πουλακάκης, Νικόλαος
Παπανδρουλάκης, Νικόλαος
Χατζηπλής, Δημήτριος
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| Abstract |
The development of new genetic tools, as well as the optimal use of existing ones to improve production in fish farming for Meagre and Greater amberjack species has been the main research goal in this doctoral dissertation. Modernization in the design of breeding programs as well as genetic selection assisted by polymorphic markers, are conditions which in their implementation give important possibilities in the production process. We developed two multiplex genetic loci for Meagre and Greater amberjack, respectively, which we used to estimate the genetic diversity of 11 stocks consisting of 946 farmed fish, to investigate the effects of stock mixing and the resulting genetic clustering via population genetics parameters. We proposed a methodology for estimating kinship relationships in multiple stocks, based on which we estimated individual inbreeding values, and we compared two methods of selecting broodstock from crosses of 27 parents from one stock. In addition, we investigated the heritability for the characters weight and length in Meagre, the genetic and phenotypic correlations of weight and length as well as the variation in the contribution of the offspring from a generation of 800 offspring to the formation of F1 families depending on effective population size. The detection of constituent elements of the genome of a species together with the description of the genetic base of important characters can contribute to the conservation of the species and the broadening of the possibilities of its productive utilization. A genetic linkage map for a species highlights genome evolution, facilitates trait mapping through QTL analysis, and improves genome assembly. We used a reduced genomic representation technique by creating a ddRAD library for six F1 families in the Meagre in a total of 266 fish, to construct a high density genetic map consisting of 4529 polymorphic SNP markers. We performed QTL analysis for weight and length, which detected a statistically significant QTL in linkage group 15. The genetic map was used for comparative genomic analysis, by finding homologs, in five genomes of teleost fish and showed a conserved evolutionarily karyotype structure in the Meagre. The observed syntenies, converged with published data, on the evolutionary proximity of the Scianidae family with the Sparidae family and specifically with the seabream, with the Moronidae family and the seabass, detected possible chromosomal rearrangement in the Meagre and especially in linkage group 17. In its entirety the dissertation provided with new genetic tools for the Meagre and Greater amberjack for the design of breeding programs. Furthermore, the first genetic linkage map of the Meagre from NGS data was contructed which was used for the genetic appreciation of the growth-related characters weight and length in the Meagre, presents with elements of evolution of the Meagre genome, and provides a high-density genetic map as a valuable tool for research and production.
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| Language |
Greek |
| Subject |
Genetic markers |
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Genetics |
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Γενετική |
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Γενετικοί δείκτες |
| Issue date |
2023-09-06 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
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Type of Work--Doctoral theses
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| Permanent Link |
https://elocus.lib.uoc.gr//dlib/a/9/3/metadata-dlib-user1691442927-32103.tkl
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| Views |
4 |
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