E-Locus - Institutional Repository of the University of Crete

Home Collections Type of Work Doctoral theses

Doctoral theses

Current Record: 1093 of 2454

[Add to Basket]

Identifier

uch.biology.phd//1995papanikolaou

Title

Ο ρόλος των μειωτών ιοντικής ισχύος στη διαλυτότητα των βιομορίων στα μικτά υδατικά ηλεκτρολυτικά διαλύματα και εφαρμογές στη χρωματογραφία και στην κρυστάλλωση

Alternative Title

The role of ionic strength reducers in the solubility of biomolecules in mixed aqueous electrolytic solutions and applications to chromatography and crystallization

Author

Παπανικολάου, Ιωάννης Κ

Thesis advisor

Γανωτάκης, Δημήτρης

Abstract

We define as Ionic strength reducers or simply reducers the organic solvents, PEG and all other substances that reduce the ionic strength of an electrolyte solution. The reducers, by decreasing the dielectric constant of a solution, induce the formation of neutral ion pairs, which results in reduced ionic strength. Green's equation describes the solubility of biomolecules in an electrolyte solution as a function of the ionic strength. We have formulated the generalized Green's equation, the solubility of biomolecules in the generalized salting in increases, when the electrolyte concentration increases and or the reducer concentration decreases and vise versa. And the solubility of macromolecules in the generalizedsaltingout increases, when the electrolyte concentration decreases and or the reducer concentration increases and vise versa. As a result of the generalized Green's equation, elution from Ion exchange matrices is included by the increase of the electrolyte concentration and or by the decrease of the reducers concentration and vise versa. In conclusion, in aqueous electrolyte solutions, the addition of ionic strength reducers increases electrostatic interactions and decreases polar interactions.

Language

Greek

Subject

Βιομόρια; Διαλυτότητα; ΕΝΑΛΑΤΩΣΗ; ΕΞΑΛΑΤΩΣΗ; Κρυστάλλωση; Οργανικοί διαλύτες; Χρωματογραφία

Issue date

1995-12-07

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses

Type of Work--Doctoral theses

Permanent Link

https://elocus.lib.uoc.gr//dlib/1/0/2/metadata-dlib-1995papanikolaou.tkl