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Identifier |
000434179 |
Title |
Ο ρόλος της RNA σίγησης στην αντίδραση υπερευαισθησίας επαγόμενη από ένα ζεύγος NLR |
Alternative Title |
The role of RNA silencing in an NLR-induced hypersensitivity response |
Author
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Αμαρτωλού, Αργυρούλα Μ.
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Thesis advisor
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Σαρρής, Παναγιώτης
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Reviewer
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Καλαντίδης, Κρίτων
Τσαγρή, Ευθυμία
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Abstract |
The aim of this study was to study the role of RNA silencing in the BnRPR1 / BnRPR2 resistance
pair-induced hypersensitivity response. Plants have developed their innate immune system, which
has been biochemically differentiated into two pathways. The first pathway, known as the basal
innate immune system, recognizes and responds to molecules common to many categories, including non-pathogens. The second, referred to as the immune system of resistance genes (ETIs),
responds to infectious agents secreted by pathogens, i.e. the operators, which are recognized by
NLRs. NLRs are intracellular receptors and promote innate immunity. Their structure consists of
the LRR domain, a NB nucleotide binding domain, and an amino-terminal domain. The regulation
of homeostasis NLRs genes are important for their function. In healthy plants, NLR proteins are
found in small amounts and / or in an inactive form. The setting of the NLR expression can occur
at several levels. One mechanism for regulating NLRs, which we will study in this paper, is that of
RNA silencing. Higher eukaryotic organisms have this RNA sequence mechanism, which results in
the inhibition of gene expression, either at the transcriptional or translational level. In N. benthamiana plants, both wild-type and those suppressed by DCL2 (dcl2), DCL4 (dcl4) and suppression of
DCL2 and DCL4 (dcl2 /4), the hypersensitivity responce and the amount of gene expression BnRPR1
/ BnRPR2, after their expression by agro-infiltration will be observed. In this way we will determine
which of the proteins DCL2 and DCL4 regulates the expression of the genes under study.
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Language |
Greek |
Subject |
BnRPR1 |
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BnRPR2 |
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DCL proteins |
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DCL πρωτεΐνες |
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Plant immune system |
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SRNA |
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Ανοσοποιητικό σύστημα |
Issue date |
2020-11-27 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/0/0/c/metadata-dlib-1606212701-508215-20451.tkl
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Views |
420 |