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Identifier |
000443928 |
Title |
In silico and in vivo characterization of the ecdysone importer Oatp74D in Lepidoptera |
Alternative Title |
In silico and in vivo ανάλυση το μεταφορέα ενδυσόνης Oatp74D στα λεπτιδόπτερα |
Author
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Φωτιάδου, Μελίνα Χ.
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Thesis advisor
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Βόντας, Ιωάννης
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Reviewer
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Παυλίδης, Παύλος
Siden-Kiamos, Inga
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Abstract |
Insect pests damage agricultural production to a great extent, imposing significant
costs to global economy. At the same time, the intensive use of insecticides often brings
about populations resistant to the existing insecticides. At present, two major lepidopteran
pests are Helicoverpa armigera and Spodoptera frugiperda. These species are considered to
be so destructive, because they are polyphagous, invasive, widely distributed and have
already developed resistance to existing insecticides via target-site mutations. Thus, the
discovery of new targets for drug development, in order to control their populations, is of
utmost importance.
Recent research in the model insect Drosophila melanogaster reports the role of a
plasma membrane transporter referred as Ecdysone Importer (EcI), also known as organic
anion transporting polypeptide 74D (Oatp74D), in the development of D. melanogaster.
Ecdysone is a steroid hormone that regulates several physiological processes. When
ecdysone enters the cell it binds and activates an intracellular nuclear receptor (EcR) which
is then able to induce the transcription of several genes essential for the development of the
organism. It was shown that EcI is necessary for ecdysone signaling. This transporter
belongs to a specific subclade of the Solute Carrier O (SLCO) family of transporters, which is
well represented in insects while being absent in mammals. These two characteristics of EcI,
the essentiality for the development of the organism and the insect specificity, render it a
potential target for the development of insecticides. However, further analysis of this
transporter is needed in pest species to prove whether it is appropriate as a drug target.
In this study, we constructed a phylogenetic tree of the SLCO transporters of several
ecdysozoa, organisms that utilize ecdysosteroids. The gene family tree provided information
on which species have potential orthologs of Oatp74D and showed that this gene probably
appeared before the evolution of arthropods, but after the evolution of ecdysozoa. After
finding the potential orthologs of Oatp74D in the pests of our interest, we proceeded to
functional characterization of the lepidopteran Oatp74D. By making use of CRISPR Cas9
system we managed to generate mosaic knockouts of Oatp74D in S. frugiperda embryos
and found that this gene is necessary for the development of the organism at embryonic and
larval stages.
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Language |
English |
Subject |
Frugiperda |
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Importer |
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Insecticides |
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Spodoptera |
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Εντομοκτόνα |
Issue date |
2021-11-26 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/9/8/0/metadata-dlib-1637842807-491027-14801.tkl
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Views |
339 |