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Identifier |
000436187 |
Title |
Biomolecular residue analysis in archaeological potsherds by means of UV-vis absorption and fluorescence spectroscopy |
Alternative Title |
Μελέτη βιομοριακών δεικτών με φασματοσκοπία απορρόφησης υπεριώδους-ορατού και φθορισμού για τον χαρακτηρισμό οργανικών καταλοίπων σε αρχαιολογικά κεραμικά |
Author
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Ιντζέ, Αντωνία Ι.
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Thesis advisor
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Άγγλος, Δημήτριος
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Reviewer
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Σπύρος, Απόστολος
Παυλίδης, Ιωάννης
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Abstract |
Bio-organic residue analysis of archaeological artefacts sheds light on human activities and
surviving practices during historic and prehistoric times using powerful analytical
methodologies based mainly on chromatographic techniques coupled with mass spectrometry.
In the context of archaeo-residue analysis, spectroscopic methods are advantageous since they
enable fast-screening of samples via prompt detection of biomarkers and, thus, expedite the
further study of promising samples by high-end analytical tools.
The present master’s thesis focuses on the study of archaeological potsherds originating from
the Minoan civilization by means of UV-vis absorption and fluorescence spectroscopy with the
aim of developing efficient detection and characterization methodologies of biomarker
compounds. Analyses of samples are conducted in solutions deriving from solvent treatment
of the sherds. The analytical methodology relies at first on recording UV-vis absorption spectra
and then on recording emission and excitation fluorescence spectra in extracted solutions
originating from three parts of the potsherds: inner, outer, and inner soil crust. Detailed
examination of fluorescent data allows the construction of three-dimensional fluorescence
excitation-emission maps for each sample and subsequently the comparison of the fluorescent
profiles of unknown samples with those of standard solutions.
Standard aqueous solutions of phenolic acids (ferulic acid, gallic acid, caffeic acid, syringic
acid, and vanillin) were prepared and studied as they comprise an important class of intrinsic
fluorophores in wine that is known to preserve over archaeological timescales. Phenolic
compounds were proposed to serve as wine markers, while secondary oxidation products of
unsaturated lipids were suggested to compose oil markers. Based on the identification of such
molecular indicators it is proposed that a Minoan amphora has been used to stored oil and wine,
while two Minoan cylinder vessels were utilised as oil containers. Fluorophores in
archaeological samples were also quantified, assuming that they were represented by phenolic
acid compounds. To this end, a comparative study to reference compounds was conducted and
it was concluded that the concentration of fluorescent molecules is about 400 μg/g of potsherd.
At the same time, a methodology for organic residue recovery from modern potsherds was
designed on the basis of intact olive oil as model sample and isooctane as extracting solvent.
The extraction efficiency of developed protocol was evaluated monitoring the intensity of
chlorophyll’s a emission band. Afterwards, two potsherds were spiked with phenolic standard
mixture and with olive oil and were transferred to a custom-built chamber for artificial aging
upon UV-irradiation. The absorption and fluorescence study of aged samples showed that nonconjugated double and triple bonds in lipid structures present in olive oils convert into
conjugated bonds in di- and tri-enes carrying carbonyl functional groups (secondary oxidation
products) and that phenolic acids photo-decompose via cleavage of acrylic acid group.
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Language |
English |
Subject |
Archaeometry |
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Artificial aging |
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Fluorescent biomarker |
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Organic residue |
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Phenolic acid |
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Potsherd |
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Αρχαιομετρία |
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Κεραμικό υπόστρωμα |
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Οργανικό κατάλοιπο |
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Τεχνητή γήρανση |
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Φαινολικό οξύ |
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Φθορίζων βιοδείκτης |
Issue date |
2021-03-26 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/6/d/6/metadata-dlib-1610368262-210549-8749.tkl
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Views |
503 |