Results - Details
Search command : Author="Γανωτάκης"
And Author="Δημήτριος"
Current Record: 18 of 101
|
Identifier |
000442170 |
Title |
Formulation and characterization of liposomes and nanoemulsions for targeted drug delivery |
Alternative Title |
Ανάπτυξη και χαρακτηρισμός λιποσωμάτων και νανογαλακτωμάτων για στοχευμένη παροχή φαρμάκων |
Author
|
Παπαβασιλείου, Μαλαματένια Γ.
|
Thesis advisor
|
Γανωτάκης, Δημήτριος
|
Reviewer
|
Χανιωτάκης, Νικόλαος
Τσιώτης, Γεώργιος
|
Abstract |
Targeted drug delivery employs carriers to transfer therapeutics mainly to tissues of
interest and to tackle issues such as drug solubility, and short circulation time. This
thesis focuses on two drug delivery systems: liposomes and nanoemulsions.
Liposomes are self-assembly structures of phospholipids, with an aqueous core,
which makes them ideal in hydrophilic drug encapsulation or hydrophobic drug
incorporation in their lipid bilayer. The aim of this study was to investigate the optimal
composition of the liposomal formulations prepared by thin-film hydration.
Conventional, stealth, and stimuli-responsive liposomes were characterized with
dynamic light scattering, electrophoretic light scattering, and microscopy, while
fluorescent dye-loaded liposomes were examined with microscopy and UV-vis
spectroscopy. Parameters that could affect either the formulation or the
characterization process were also evaluated. Depending on the formulation’s
ingredients, liposomal sizes ranged from 150 to 300 nm. The liposomes also
exhibited low polydispersity and good stability during storage.
Nanoemulsions are also a promising alternative; they consist of an oily core and a
surfactant monolayer. Two types of non-ionic surfactants were examined, a mixture
of Span80/Tween20 as well as Kolliphor HS 15. The phase inversion temperature
method was used for the nanoemulsion preparation. Dynamic light scattering,
electrophoretic light scattering, and microscopy were employed for the
characterization of the nanoemulsion droplets size, zeta-potential, polydispersity,
and morphology. The choice of the surfactant affected immensely the characteristics
of the nanoemulsion. The average diameter of the nanoemulsions ranged from 40
nm to 120 nm. Apart from empty nanoemulsions, a reverse micelle-loaded
nanoemulsion was also prepared.
This work includes an extensive literature review, an attempt to answer the questions
that arose during the experiments, and an evaluation of the limitations of the
presented methodologies.
|
Language |
English |
Subject |
Drug delivery systems |
|
Dynamic light scattering |
|
Fluorescence microscopy |
|
Phase inversion temperature |
|
Thin-film hydration |
|
Δυναμική σκέδαση φωτός |
|
Ενυδάτωση λεπτής μεμβράνης |
|
Θερμοκρασία αναστροφής φάσης |
|
Μικροσκοπία φθορισμού |
|
Συστήματα μεταφοράς φαρμάκων |
Issue date |
2021-11-24 |
Collection
|
School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
|
|
Type of Work--Post-graduate theses
|
Permanent Link |
https://elocus.lib.uoc.gr//dlib/c/8/0/metadata-dlib-1632220895-584030-16712.tkl
|
Views |
299 |
Digital Documents
|
|
No permission to view document.
It won't be available until: 2024-11-24
|